JCRB0612.1 GOTO.P3

Cell information

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GOTO.P3について

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0612.1 Cell Name   GOTO.P3
Profile   Serum free cell culture of neuroblastoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   13-month-old
Identity    Tissue for Primary Cancer   neural
Case history   neuroblastoma apeared in the left adrenal gland. Metastasis   
Tissue Metastasized    Genetics   neroblastoma
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   GOTO cells cultured with serum free medium for long period by serial subcultures.
Classify   tumor Established by   Takaoka,T. & Sekiguchi,M.
Registered by   Takaoka,T. Regulation for Distribution   
Comment    Year   2004
Medium   Serum free DM201 medium. Methods for Passages   Cells are harvested by rubber policeman gently.
Cell Number on Passage   10^4 cells/sq.cm. Race   Japanese
CO2 Conc.    Tissue Sampling   adrenal grand
Tissue Type   neuroblastoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2911
Reference
Pubmed id:470188Establishment and characterization of a human neuroblastoma cell line in tissue culture.
Sekiguchi M,Oota T,Sakakibara K,Inui N,Fujii G
Jpn J Exp Med. 1979 Feb;49(1):67-83

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LOT Information

Cell No.   JCRB0612.1 Cell Name   GOTO.P3
LOT No.   08272004 Lot Specification   distribution
Medium   DM201 medium. Serum Free. Temperature   37 C
Cell Density at Seeding   1.0-2.5x10^4 cells/sq.cm. Methods for Passages   Harvest cells with scraper. Subculture every 7-10 days and medium change every 2-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   71.5 Antibiotics Used   MC210 treated on this culture only. (3 weeks).
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:15
D7S820:8,11
D16S539:13
VWA:17,19
TH01:9
AM:X
TPOX:11
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   1 to 1 mixture of the culture medium and the FM-1 medium containing 10% DMSO(final). CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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