JCRB0618.1 TGW.P3

Cell information

Important Notice(s)

Mycoplasmal contamination of JCRB0618.1 TGW.P3

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0618.1 Cell Name   TGW.P3
Profile   Serum free cell culture of the human neuroblastoma cell line, TGW. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   23
Identity   available Tissue for Primary Cancer   neural
Case history   disseminated neuroblastoma. Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Sekiguchi,M.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment    Year   2004
Medium   Serum free DM201 medium. Methods for Passages   Cells are harvested by rubber policeman gently.
Cell Number on Passage    Race   Japanese
CO2 Conc.    Tissue Sampling   adrenal grand
Tissue Type   neuroblastoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3180
Reference
Pubmed id:2335222Origin of human neuroblastoma cell lines TGW and TNB1.
Tsuchida Y,Sekiguchi M,Kaneko Y,Kanda N
FEBS Lett. 1990 Apr 24;263(2):191

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LOT Information

Cell No.   JCRB0618.1 Cell Name   TGW.P3
LOT No.   12212004 Lot Specification   distribution
Medium   DM201 serum free medium. Temperature   37 C
Cell Density at Seeding   3.4 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested by pipetting with EDTA-PBS. Subculture once a week and medium change every 2-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   88.6 Antibiotics Used   free
Passage Number   P9* PDL   NT
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   One to one mix of the DM201 and FM-1 containing 10% DMSO finally. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0618.1 Cell Name   TGW.P3
LOT No.   08262004 Lot Specification   distribution
Medium   DM201 medium. Serum free. Temperature   37 C
Cell Density at Seeding   4.5 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested by pipett or scraper. Subculture every 8-14 days and medium change every 2-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   62.9 Antibiotics Used   Free. MC210 temporary added to remove mycoplasmas.
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa..
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   1 to 1 mixture of the culture medium and the FM-1 medium containing 10% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
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