JCRB0152.3 M.P3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0152.3 Cell Name   M.P3
Profile   M cells derived from RLC-3 (JAR rat) was habituated to serum free culture medium. Other Name   
Animal   rat Strain   JAR-1
Genus   Rattus Species   norvegicus
Sex    Age   10-day-old
Identity   not done Tissue for Primary Cancer   liver
Case history   normal Metastasis   
Tissue Metastasized    Genetics   4-dimethylaminoazobenzene(DAB) transformed and remarkable changes were found in the biochemical properties.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Morphological changes observed cell line by DAB treatment. Historical cell line produced by Nagisa culture and DAB treatment.
Classify   transformed Established by   Katsuta,H. and Takaoka,T.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment   Cells grow in serum free medium established by Takaoka,T. and Katsuta,H. Year   2004
Medium   DM201 medium. (This culture requires serum free medium definitely). Methods for Passages   Cells harvested by scraper and hard to individualize cells. Additional information is under preparation.
Cell Number on Passage   Split ratio = 1/2. Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   tumor created by DAB treatment with Nagisa cu

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5551
Reference
Pubmed id:7441906An attempt in tissue culture at preventing and treating the collagen fiber formation of liver cells.
Katsuta H,Takaoka T
Jpn J Exp Med. 1980 Aug;50(4):275-82

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LOT Information

Cell No.   JCRB0152.3 Cell Name   M.P3
LOT No.   09132004 Lot Specification   distribution
Medium   DM201 medium. No serum added. Temperature   37 C
Cell Density at Seeding   7-8 x 10^3 cells/sq.cm. Methods for Passages   Cells harvested with scraper. Subculture every 7-10 days and medium change every 2-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   76.9 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   1 to 1 mixture of the culture medium and the FM-1 medium. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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