JCRB0266 PC12HS

Cell information

Important Notice(s)

PC12HS, MS, LSについて

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0266 Cell Name   PC12HS
Profile   Highly NGF sensitive clone of rat PC12 cell line. Other Name   PC12 clone HS
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   pheochromocytoma of the adrenal medulla. Metastasis   No
Tissue Metastasized    Genetics   NGF-highly sensitive clone (HS)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Cells are differentiated into sympathetic neuron-like extentions when they are exposed to NGF.
Classify   variant Established by   Iizuka,H. & Umeda,M.
Registered by   Umeda,M. Regulation for Distribution   
Comment    Year   1997
Medium   RPMI1640 medium with 10% heat inactivated horse serum and 5% fetal bovine serum. Methods for Passages   Cells were harvested with pipetting.
Cell Number on Passage   3-5 x 10^4 cells/sq.cm. Race   
CO2 Conc.   5 % Tissue Sampling   chromaffin cell
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J438
Reference
Pubmed id:1493541Different ability in PC12 clones to recover from MMC toxicity following NGF treatment.
Iizuka H,Umeda M
Brain Res. 1992 Dec 18;599(1):1-5
Pubmed id:3839317Molecular cloning of a gene sequence regulated by nerve growth factor.
Levi A,Eldridge JD,Paterson BM
Science. 1985 Jul 26;229(4711):393-5
Pubmed id:6641712A macromolecular structure favouring microtubule assembly in NGF-differentiated pheochromocytoma cells (PC12).
Biocca S,Cattaneo A,Calissano P
EMBO J. 1983;2(5):643-8
Pubmed id:742018[Therapeutic results in adult acute leukemia in Giessen].
Löffler H,Pralle H,Graubner M,Schrezenmaier E
Verh Dtsch Ges Inn Med. 1978;(84):156-8
Pubmed id:1065897Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Greene LA,Tischler AS
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8

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LOT Information

Cell No.   JCRB0266 Cell Name   PC12HS
LOT No.   03232011 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated horse serum and 5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   vigorous pipetting.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.4x 10^6
Viability at cell freezing (%)   92.9 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   Expected viability (defrost ampoule) 93 %.
Cell No.   JCRB0266 Cell Name   
LOT No.   06212007 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% horse serum and 5% fetal bovine serum Temperature   37 C
Cell Density at Seeding   2-4 x 10^5 cells/ml Methods for Passages   Pipetting. The cells loosely attach to the substratum and often form cell clumps.
Doubling Time   approx. 2-3 days Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   77 Antibiotics Used   free
Passage Number   Unknown (9 at cellbank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture medium containing 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0266 Cell Name   PC12HS
LOT No.   11222004 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated horse serum and 5% fetal bovine serum. Temperature   
Cell Density at Seeding   3.0 x 10^4 cells / sq.cm Methods for Passages   Cells are harvested by pipetting.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.52x10^6
Viability at cell freezing (%)   88.1 Antibiotics Used   NT
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0266 Cell Name   PC12HS
LOT No.   02132019 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% heat-inactivated horse serum (GIBCO Cat # 26050) and 5% fetal bovine serum (Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.9 - 1.6 x 10^5 cells/mL Methods for Passages   The cells were harvested by vigorous pipetting.
Doubling Time   approx. 34 hrs. Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   68.5 Additional information   
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