JCRB0607.1 MK.P3
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0607.1 | Cell Name | MK.P3 |
|---|---|---|---|
| Profile | Serum free cell culture of the MK(monkey kidney, JTC-12) cell. | Other Name | JTC-12.P3 |
| Animal | monkey, Cynomolgus | Strain | Cynomologus |
| Genus | Macaca | Species | fascicularis |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | kidney | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | kidney | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epitherial-like | Character | Adapted to serum free DM-160 medium and maintained by Takaoka,T. for long passages by continuous cultuivation without freezing. |
| Classify | deposit | Established by | Katsuta,H. |
| Registered by | Takaoka,T. | Regulation for Distribution | |
| Comment | Usage of serum cause changes of the cell characetristics. Serum free medium is essential to keep the characteristics of this cell line. JCRB0607 will be eliminated and the NIHS0354 will be opened for the distribution. | Year | 2004 |
| Medium | DM160 medium. No serum required. | Methods for Passages | Harvest cells with rubber policeman. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | kidney |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
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| Pubmed id:7905235 | Propagation of hepatitis A virus in a renal cell line JTC-12.P3 of cynomolgus monkey origin. Baba M,Takegawa M,Kaito M,Miyamoto K,Suzuki S Acta Virol. 1993 Aug;37(4):209-22 |
| Pubmed id:3142784 | A new approach to the modification of cell membrane glycosphingolipids: ganglioside composition of JTC-12 P3 cells altered by feeding with galactose as a sole carbohydrate source in protein- and lipid-free synthetic medium. Kawaguchi T,Takaoka T,Yoshida E,Iwamori M,Takatsuki K,Nagai Y Exp Cell Res. 1988 Dec;179(2):507-16 |
| Pubmed id:3530606 | Regulation of sodium-coupled phosphate transport by extracellular phosphate in cultured kidney cells (JTC-12). Takuwa Y,Takeuchi Y,Ogata E Clin Sci (Lond). 1986 Sep;71(3):307-12 |
| Pubmed id:3933482 | Characterization of Na+-dependent phosphate uptake in cultured kidney cells (JTC-12) from monkey. Takuwa Y,Ogata E Biochem J. 1985 Sep 15;230(3):715-21 |
| Pubmed id:2863248 | Differentiated properties characteristic of renal proximal epithelium in a cell line derived from a normal monkey kidney (JTC-12). Takuwa Y,Ogata E In Vitro Cell Dev Biol. 1985 Aug;21(8):445-9 |
| Pubmed id:208643 | Hormone-specific responses and biosynthesis of sulfolipids in cell lines derived from mammalian kidney. Ishizuka I,Tadano K,Nagata N,Niimura Y,Nagai Y Biochim Biophys Acta. 1978 Jul 17;541(4):467-82 |
| Pubmed id:351092 | [Topics on sulfated glycolipids (author's transl)]. Ishizuka I Seikagaku. 1978 Mar 25;50(3):145-59 |
| Pubmed id:13984589 | Establishment of a cell strain, JTC-12, from cynomolgus monkey kidney tissue. TAKAOKA T,KATSUTA H,ENDO M,SATO K,OKUMURA H Jpn J Exp Med. 1962 Aug;32():351-68 |
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LOT Information
| Cell No. | JCRB0607.1 | Cell Name | MK.P3 |
|---|---|---|---|
| LOT No. | 10052004 | Lot Specification | distribution |
| Medium | DM201 (serum-free medium) | Temperature | 37 C |
| Cell Density at Seeding | 1.5 x 10^4 / sq.cm | Methods for Passages | Cells harvested with scraper. Subculture 10-14 days, medium change every 3-4 days. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.38x10^6 |
| Viability at cell freezing (%) | 73.3 | Antibiotics Used | NT |
| Passage Number | P10* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | One to one mix with Cultue medium and FM-1(containing 10%DMSO) medium. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
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