JCRB1086 KYSE220

Cell information

Important Notice(s)

KYSEシリーズ細胞株の民間企業への分譲について

On the MTA of KYSE series cell lines with Kyoto University (for profit organizations)

KYSEシリーズ細胞株の使用について

Announcement of unavailability of KYSE series cell lines to oversea users outside of Japan

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1086 Cell Name   KYSE220
Profile   Human squamous cell carcinoma from esophagus. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   78
Identity   available Tissue for Primary Cancer   esophagus
Case history   esophagus cancer Metastasis   No
Tissue Metastasized    Genetics   amplification of int-2 and hst-1 observed, Species were verified by HLA analysis
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   T3N1M0 Stage 3. Both p53 and p16 mutations were observed. No virus infections were observed.
Classify   tumor Established by   Shimada,Y.
Registered by   Shimada,Y. Regulation for Distribution   Cite reference {5368}. Company users require MTA from the originator. See additional information.
Comment   Distribution restricted to Japan(domestic use only). Year   2004
Medium   1 to 1 mix of Ham's F12 and RPMI1640 medium containing 2% fetal calf serum. Methods for Passages   
Cell Number on Passage   split ratio = 1/5 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   esophagus
Tissue Type   Moderately differenciated SCC

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1359
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240167
Reference
Pubmed id:11092977Nonrandom chromosomal imbalances in esophageal squamous cell carcinoma cell lines: possible involvement of the ATF3 and CENPF genes in the 1q32 amplicon.
Pimkhaokham A,Shimada Y,Fukuda Y,Kurihara N,Imoto I,Yang ZQ,Imamura M,Nakamura Y,Amagasa T,Inazawa J
Jpn J Cancer Res. 2000 Nov;91(11):1126-33
Pubmed id:10446988Lymph node metastasis is associated with allelic loss on chromosome 13q12-13 in esophageal squamous cell carcinoma.
Harada H,Tanaka H,Shimada Y,Shinoda M,Imamura M,Ishizaki K
Cancer Res. 1999 Aug 1;59(15):3724-9
Pubmed id:9699676Methylation of the 5' CpG island of the FHIT gene is closely associated with transcriptional inactivation in esophageal squamous cell carcinomas.
Tanaka H,Shimada Y,Harada H,Shinoda M,Hatooka S,Imamura M,Ishizaki K
Cancer Res. 1998 Aug 1;58(15):3429-34
Pubmed id:9033652Multiple types of aberrations in the p16 (INK4a) and the p15(INK4b) genes in 30 esophageal squamous-cell-carcinoma cell lines.
Tanaka H,Shimada Y,Imamura M,Shibagaki I,Ishizaki K
Int J Cancer. 1997 Feb 7;70(4):437-42
Pubmed id:8575860Characterization of p53 gene mutations in esophageal squamous cell carcinoma cell lines: increased frequency and different spectrum of mutations from primary tumors.
Tanaka H,Shibagaki I,Shimada Y,Wagata T,Imamura M,Ishizaki K
Int J Cancer. 1996 Jan 26;65(3):372-6
Pubmed id:9816044p53 mutation, murine double minute 2 amplification, and human papillomavirus infection are frequently involved but not associated with each other in esophageal squamous cell carcinoma.
Shibagaki I,Tanaka H,Shimada Y,Wagata T,Ikenaga M,Imamura M,Ishizaki K
Clin Cancer Res. 1995 Jul;1(7):769-73
Pubmed id:7913084Analysis of gene amplification and overexpression in human esophageal-carcinoma cell lines.
Kanda Y,Nishiyama Y,Shimada Y,Imamura M,Nomura H,Hiai H,Fukumoto M
Int J Cancer. 1994 Jul 15;58(2):291-7
Pubmed id:8518899Prognostic significance of cell culture in carcinoma of the oesophagus.
Shimada Y,Imamura M
Br J Surg. 1993 May;80(5):605-7
Pubmed id:1728357Characterization of 21 newly established esophageal cancer cell lines.
Shimada Y,Imamura M,Wagata T,Yamaguchi N,Tobe T
Cancer. 1992 Jan 15;69(2):277-84

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LOT Information

Cell No.   JCRB1086 Cell Name   
LOT No.   04152008 Lot Specification   distribution
Medium   1:1 mixture of Ham's F12 medium and RPMI 1640 medium with 2% fetal bovine serum (FBS Lot; GIBCO Cat. No. 10099, Lot 484375) Temperature   37 C
Cell Density at Seeding   0.6 - 1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA at 37 C for 5 min.
Doubling Time   approx. 16 hrs Cell Number in Vial (cells/1ml)   6.3 x 10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   P287 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1086 Cell Name   KYSE220
LOT No.   10122004 Lot Specification   distribution
Medium   Mixed medium(1/1) of the RPMI1640 and the F12 medium containing 2% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   2.7 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin. Subculture once a week and medium change every 2-3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   P285 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5 % DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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