JCRB1088 RI-T
Cell information
Important Notice(s)Mycoplasma elimination by Plasmocin treatment(JCRB1088:RI-T Lot 07122013)
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1088 | Cell Name | RI-T |
|---|---|---|---|
| Profile | Rat hepatic stellate cell(HSC) line immortalized with SV40 T antigen. | Other Name | |
| Animal | rat | Strain | Wistar |
| Genus | Rattus | Species | norvegicus |
| Sex | M | Age | adult |
| Identity | Tissue for Primary Cancer | liver | |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | SV40 antigen positive, desmin, and type IV collagen were detected in cytoplasms. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | spindle-shape | Character | The cells were established by the transfection of a pRSV-T. The cell line has both morphologilal and functional characteristics of HSC. |
| Classify | transformed | Established by | Tokiwa,T. and Yata,T. |
| Registered by | Tokiwa,T. | Regulation for Distribution | |
| Comment | Early subculture is recommended when sustantial spaces are seen between cells under microscope. | Year | 2004 |
| Medium | William's E medium with 10^-6M dexamethasone, 10^-7M insulin, 25ng/ml EGF, and 10% fetal bovine serum. or RPMI-1640 medium with 10% fetal bovine serum. | Methods for Passages | Cells were prepared after treatment with 0.05% trypsin and 0.53mM EDTA-4Na for 3-5 min. Subculture every 4-6 days and medium change every 2-3 days. Lower concentration of cells on subculture may create colonies. |
| Cell Number on Passage | split ratio = 1/2 | Race | |
| CO2 Conc. | Tissue Sampling | liver perfused by 0.05% collagenase | |
| Tissue Type | hepatic stellate cell (pSV40 TAg) |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:10475269 | SV40 large T antigen immortalization of rat hepatic stellate-like cells. Tokiwa T,Yata Y,Takahara T,Watanabe A,Enosawa S,Suzuki S,Kano J,Noguchi M In Vitro Cell Dev Biol Anim. 1999 May;35(5):246-7 |
| Pubmed id:none | Characterization of immortalized rat hepatic stellate cell lines transfected with SV40 T antigen Yata,Y., Tokiwa,T., Takahara,T., Zhang,L.P., and Watanabe,A. Hepatology Research 1999; 14(3):239-44 |
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LOT Information
| Cell No. | JCRB1088 | Cell Name | RI-T |
|---|---|---|---|
| LOT No. | 07122013 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.3-1.3x10^5 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.26x10^6 |
| Viability at cell freezing (%) | 97.7 | Antibiotics Used | |
| Passage Number | p26 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | PCR(species), rat |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1088 | Cell Name | |
|---|---|---|---|
| LOT No. | 11272008 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. No. 10099, Lot 484375) | Temperature | 37 C |
| Cell Density at Seeding | approx. 5 x 10^4 cells/ml | Methods for Passages | 0.05% trypsin and 0.02% EDTA in PBS (=) at room temp for 2 min. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.1 x 10^6 | |
| Viability at cell freezing (%) | 95.5 | Antibiotics Used | 50 ug/ml kanamycin |
| Passage Number | P30 | PDL | |
| Sterility: MYCOPLASMA | + | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as rat by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1088 | Cell Name | RI-T |
|---|---|---|---|
| LOT No. | 12112004 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% fetal bovine serum (Intergen RB52405). | Temperature | 37C |
| Cell Density at Seeding | 2.8x10^4cells/sq.cm. | Methods for Passages | Cells are harvested after treatment with 0.05% trypsin and 0.02% EDTA at room temperature for 2 minutes. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.8 x 10^6 |
| Viability at cell freezing (%) | 91% | Antibiotics Used | Free |
| Passage Number | P26 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium containing 5% DMSO. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
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