Cell information

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Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1091 Cell Name   NCE SVIIA3
Profile   SV40 transformed keratinocyte cells derived from human cervix. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   adult
Identity   available Tissue for Primary Cancer   cervix
Case history    Metastasis   
Tissue Metastasized    Genetics   SV40 ori(-), teromerase positive, P75NTR positive.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   SV40 transformed human keratinocyte from cervix.
Classify   transformed Established by   Yasumoto,S.
Registered by   Yasumoto,S. Regulation for Distribution   Not apllied.
Comment    Year   2001
Medium   MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml bovine pituitary extract. Methods for Passages   Cells are harvested with 0.1% trypsin and 0.1% EDTA. Medium change every 2 days and subculture every 10 days.
Cell Number on Passage   1.5 - 2.4 x 10^5 cells/sq.cm. Race   Japanese
CO2 Conc.    Tissue Sampling   cervix
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8374
Pubmed id:12747968Gene expression of telomerase related proteins in human normal oral and ectocervical epithelial cells.
Fujimoto R,Kamata N,Taki M,Yokoyama K,Tomonari M,Nagayama M,Yasumoto S
Oral Oncol. 2003 Jul;39(5):445-52


LOT Information

Cell No.   JCRB1091 Cell Name   
LOT No.   11102005 Lot Specification   distribution
Medium   MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10ug/ml transferrin, 0.1mM phosphoethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF, 50 ug/ml BPE (serum-free). Temperature   37 C
Cell Density at Seeding   2.7 - 5.9 x 10^4 cells/ml Methods for Passages   0.025% trypsin and 0.02% EDTA. Trypsin activity was stopped with soybean trypsin inhibitor.
Doubling Time   approx. 3 days Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   54.3 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellulose - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information