JCRB1093 PSVK1

Cell information

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Supplements for the Serum free culture medium

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1093 Cell Name   PSVK1
Profile   Human normal keratinocyte prepared from human foreskin that was immortalized by the infection of the pSV40 ori-(minus) DNA. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   0-year-old
Identity   available Tissue for Primary Cancer   foreskin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   Cells transfected by SV40 ori- (minus) DNA. Teromerase activity is maintained.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Human primary cell line immortalized by pSV40 gene.
Classify   transformed Established by   Yasumoto,S.
Registered by   Yasumoto,S. Regulation for Distribution   not applied
Comment    Year   2000
Medium   MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml bovine pituitary extract. (serum-free) Methods for Passages   Cells are harvested with 0.1% trypsin and 0.1% EDTA. Medium change every 2 days and subculture every 10 days.
Cell Number on Passage   1.5 - 2.4 x 10^5 cells/sq.cm. Race   
CO2 Conc.    Tissue Sampling   foreskin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8403
Reference
Pubmed id:none Telomere dynamics and telomerase activities during immortalization of human epithelial cells transfected with HPV16 and SV40 oncogenes.
Ohji,H.
Yokohama Igaku, 49: 35-43, 1998.

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LOT Information

Cell No.   JCRB1093 Cell Name   PSVK1
LOT No.   01052005 Lot Specification   distribution
Medium   MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml bovine pituitary extract. (serum-free) Temperature   37 C
Cell Density at Seeding   1.0 x 10^4/sq. cm Methods for Passages   Cells harvested after treatment with 0.1% trypsin and 0.1% EDTA. Subculture once a week, medium change every 2-3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.77x10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   P32 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:12
D7S820:10,11
D16S539:9,13
VWA:16
TH01:6,7
AM:X,Y
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   One to one mix with Cultue medium and FM-1(containing 10%DMSO) medium. CO2 Conc.   5%
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1093 Cell Name   PSVK1
LOT No.   11262014 Lot Specification   distribution
Medium   KGM-Gold (Lonza Cat.# 00192060). Originally the instructed medium was MCDB153 medium with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml BPE. Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/mL Methods for Passages   Cells are harvested with 0.1% trypsin and 0.02% EDTA. Trypsin activity was terminated by 0.1% (final conc.) soybean trypsin inhibitor (Sigma type IS) after trypsin treatment.
Doubling Time    Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   93.2 Antibiotics Used   free
Passage Number   P36 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G5PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   0.1% methylcellulose, 10% DMSO - KGM-Gold. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93.2
Additional information   
Cell No.   JCRB1093 Cell Name   PSVK1
LOT No.   10272016 Lot Specification   distribution
Medium   KGM-Gold (Lonza) Temperature   37 C
Cell Density at Seeding   1.2 - 1.6 x 10^5 cells/mL Methods for Passages   Cells are harvested with 0.1% trypsin and 0.1% EDTA. Trypsin action was terminated by 0.1% soybean trypsin inhibitor (Sigma, type IS).
Doubling Time   59 - 126 hrs. Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   89.7 Antibiotics Used   free
Passage Number   P35 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 0.1% methylcellurose in culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.7
Additional information   
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