JCRB0246 RC-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0246 Cell Name   RC-1
Profile   cornea, (white rabbit) Other Name   
Animal   rabbit Strain   white
Genus    Species   
Sex    Age   
Identity    Tissue for Primary Cancer   cornea
Case history    Metastasis   
Tissue Metastasized    Genetics   cornea
Life Span    Crisis PDL   
Morphology    Character   cornea
Classify    Established by   Watanabe,M.
Registered by   M.Watanabe Regulation for Distribution   
Comment    Year   1988
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   Cells are treated with 0.1 % trypsin
Cell Number on Passage   10^6 cells/T75 flask Race   
CO2 Conc.   5 % Tissue Sampling   cornea
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2705
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LOT Information

Cell No.   JCRB0246 Cell Name   RC-1
LOT No.   05142013 Lot Specification   distribution
Medium   MEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.0-4.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.18x10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   p18 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   PCR(species), Rabbit
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB0246 Cell Name   
LOT No.   04142003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca. 60 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^5
Viability at cell freezing (%)   85.1 Antibiotics Used   free
Passage Number   P16 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rabbit by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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