JCRB1094 SUIT-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1094 Cell Name   SUIT-2
Profile   Human pancreatic cancer cell line established from liver metastasis. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   73
Identity   available Tissue for Primary Cancer   pancreas
Case history   pancreatic cancer (entire pancreas and extended into other tissues, blood group was A). Metastasis   Yes
Tissue Metastasized    Genetics   Mode of chromosome=45 (34-176)
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   PDL=38.2 hrs
Classify   tumor Established by   Iwamura,T. et al.
Registered by   Iguchi,H. Regulation for Distribution   
Comment    Year   2003
Medium   Eagle's minimal essential medium with 10% fetal bovine serum was used at the establishment of cells. Current medium for prpagation is RPMI 1640 medium with 10% FBS. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin in PBS(-). Subculture once a week.
Cell Number on Passage   1/8 - 1/32 splits. Race   Japanese
CO2 Conc.   5 % Tissue Sampling   metastatic liver tumor
Tissue Type   moderately differentiated tubular adenocarcin

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3172
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 1240219
Reference
Pubmed id:3130361Immunohistochemistry of human gastrointestinal cancer-associated antigen detected by monoclonal antibody PA 8-15.
Yoshimura R,Sakamoto K,Egami H,Akagi M
Jpn J Cancer Res. 1988 Feb;79(2):255-63
Pubmed id:2445616Kinetics of carcinoembryonic antigen and carbohydrate antigen 19-9 production in a human pancreatic cancer cell line (SUIT-2).
Iwamura T,Katsuki T
Gastroenterol Jpn. 1987 Oct;22(5):640-6
Pubmed id:3102439Establishment and characterization of a human pancreatic cancer cell line (SUIT-2) producing carcinoembryonic antigen and carbohydrate antigen 19-9.
Iwamura T,Katsuki T,Ide K
Jpn J Cancer Res. 1987 Jan;78(1):54-62

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LOT Information

Cell No.   JCRB1094 Cell Name   
LOT No.   09252012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1.0 - 5.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   29 - 33 hrs Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   89.6 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1094 Cell Name   SUIT-2
LOT No.   08022010 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   3.46-5.45X10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.28x10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,11
D7S820:9,11
D16S539:9
VWA:16,17
TH01:6
AM:X
TPOX:8,10
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1094 Cell Name   
LOT No.   09152009 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^5 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   20 hrs. Cell Number in Vial (cells/1ml)   4.5 x 10^6
Viability at cell freezing (%)   91.2 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1094 Cell Name   SUIT-2
LOT No.   01182007 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot;GIBCO 4218333S). Temperature   37 C
Cell Density at Seeding   1.1-1.7x10^5 cells/ml Methods for Passages   Cells are treaed with 0.25% trypsin and 0.02% EDTA before passage. Passage twice a week.
Doubling Time   Td = 48 hrs Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   free
Passage Number   P7* PDL   UNK
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD(typeB), MD and LD.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1094 Cell Name   SUIT-2
LOT No.   04122021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.4 - 4.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   99.5 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   97.8 Additional information   
Cell No.   JCRB1094 Cell Name   SUIT-2
LOT No.   03172023 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012,　Lot#12J396) Temperature   37 C
Cell Density at Seeding   3.04-3.23x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.04x10^6
Viability at cell freezing (%)   97.00 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,11
D7S820:9,11
D16S539:9
VWA:16,17
TH01:6
AM:X
TPOX:8,10
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94.00 Additional information   
Images
Cell No.   JCRB1094 Cell Name   SUIT-2
LOT No.   02162024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.6 - 4.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   4.9 x 10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   99 Additional information   
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