JCRB1096 hs-103-3

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1096 Cell Name   hs-103-3
Profile   Temperature sensitive mutant of Chinese hamster lung cell line. Other Name   
Animal   hamster, Chinese Strain   Chinese
Genus   Cricetulus Species   griseus
Sex    Age   
Identity    Tissue for Primary Cancer   lung
Case history   normal, MNNG treated Metastasis   
Tissue Metastasized    Genetics   Temperature sensitive mutant, which are transformed below 34.5 C(heat sensitive).
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   anchorage independent cell line treated by MNNG
Classify   mutant Established by   Miyashita,K.
Registered by   Miyashita,K. Regulation for Distribution   
Comment    Year   2005
Medium   Ham F12 medium with 5% Fetal bovine serum. Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   fibroblast

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8549
Reference
Pubmed id:8168155 Insulin stimulates colony formation at a nonpermissive temperature by thermosensitive mutants of Chinese hamster lung cells that exhibit anchorage-independent growth in soft-agar culture.
Miyashita K
Cell Struct Funct. 1993 Oct;18(5):303-14
Pubmed id:1295695 Calcium-sensitive, actin-related gelation of cell extracts of thermosensitive Chinese hamster lung cells capable of anchorage-independent growth.
Miyashita K
Cell Struct Funct. 1992 Dec;17(6):395-405
Pubmed id:2437133 Inhibition by cytochalasin B of DNA synthesis in a thermosensitive anchorage-independent growth mutant.
Miyashita K
J Cell Physiol. 1987 Apr;131(1):99-106
Pubmed id:1170023 Isolation of heat- and cold-sensitive mutants of chinese hamster lung cells affected in their ability to express the transformed state.
Miyashita K,Kakunaga T
Cell. 1975 Jun;5(2):131-8

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LOT Information

Cell No.   JCRB1096 Cell Name   hs-103-3
LOT No.   04282005 Lot Specification   distribution
Medium   Ham's F12 medium with 5% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   3.5x10^4sq.cm. Methods for Passages   Harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   99.4 Antibiotics Used   Free
Passage Number   P5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined.Chinese hamster
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   culture medium with 5% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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