JCRB1108 UCB408E6E7-31
Cell information
Important Notice(s)不死化していないヒト体性幹細胞の分譲について(倫理的な取り扱い)
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1108 | Cell Name | UCB408E6E7-31 |
|---|---|---|---|
| Profile | Human umbilical cord blood-derived mesenchymal stem cells. Not immortalized cell line (limited life span). | Other Name | |
| Animal | human | Strain | |
| Genus | Species | ||
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | mesenchymal stem cell |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Human umbilical cord blood-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the E6 and E7. | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | ** |
| Classify | normal | Established by | Umezawa, A. |
| Registered by | Umezawa, A. | Regulation for Distribution | |
| Comment | Year | 2005 | |
| Medium | Plusoid-M | Methods for Passages | When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells. |
| Cell Number on Passage | -1 x 10^5 cells / 100-mm dish | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | umbilical cord blood |
| Tissue Type | umbilical cord blood |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:15647378 | Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway. Terai M,Uyama T,Sugiki T,Li XK,Umezawa A,Kiyono T Mol Biol Cell. 2005 Mar;16(3):1491-9 |
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LOT Information
| Cell No. | JCRB1108 | Cell Name | UCB408E6E7-31 |
|---|---|---|---|
| LOT No. | 08052005 | Lot Specification | distribution |
| Medium | PLUSOID-M(complete medium) | Temperature | 37 C |
| Cell Density at Seeding | 2.0x10^2 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.0x10^6 |
| Viability at cell freezing (%) | 95.2 | Antibiotics Used | Free |
| Passage Number | P9 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), NP, LD examined. Human, not HeLa |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,13 D13S317:9,11 D7S820:8,10 D16S539:9,10 VWA:17,18 TH01:7,9 AM:X,Y TPOX:9,11 CSF1PO:11 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker:BLC-1 | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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