JCRB1110 UCB408E6E7TERT-33

Cell information

Important Notice(s)

PLUSOID-M medium

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1110 Cell Name   UCB408E6E7TERT-33
Profile   Human umbilical cord blood-derived mesenchymal stem cells. Other Name   
Animal   human Strain   
Genus    Species   
Sex    Age   
Identity   available Tissue for Primary Cancer   mesenchymal stem cell
Case history    Metastasis   No
Tissue Metastasized    Genetics   Human umbilical cord blood-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the E6, E7, and hTERT.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   **
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2005
Medium   Plusoid-M Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Cell Number on Passage   -1 x 10^5 cells / 100-mm dish Race   Japanese
CO2 Conc.   5% Tissue Sampling   umbilical cord blood
Tissue Type   umbilical cord blood

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3231
Reference
Pubmed id:15647378 Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway.
Terai M,Uyama T,Sugiki T,Li XK,Umezawa A,Kiyono T
Mol Biol Cell. 2005 Mar;16(3):1491-9

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LOT Information

Cell No.   JCRB1110 Cell Name   
LOT No.   10142011 Lot Specification   distribution
Medium   PLUSOID M medium (GP Biosciences, Cat. # 4013) Temperature   37 C
Cell Density at Seeding   2.8 - 5.8 x 10^4 cells/mL Methods for Passages   Cells were treated after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   6.5 x 10^5
Viability at cell freezing (%)   82.3 Antibiotics Used   not added.
Passage Number   P28 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO - PLUSOID M CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1110 Cell Name   UCB408E6E7TERT-33
LOT No.   04062009 Lot Specification   distribution
Medium   PLUSOID-M(complete medium) Temperature   37 C
Cell Density at Seeding   4.6x10^3 cells/sq.cm Methods for Passages   When the cultures reached subconfluence,the cells were harvested after treatment with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   NT
Passage Number   p27 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
D13S317:9,11
D7S820:8,10
D16S539:9,10
VWA:17,18
TH01:7,9
AM:X,Y
TPOX:9,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1110 Cell Name   UCB408E6E7TERT-33
LOT No.   05262008 Lot Specification   distribution
Medium   PLUSOID-M(complete medium) catalog No.4013 Temperature   37 C
Cell Density at Seeding   3.7x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   93.9 Antibiotics Used   NT
Passage Number   p28 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
D13S317:9,11
D7S820:8,10
D16S539:9,10
VWA:17,18
TH01:7,9
AM:X,Y
TPOX:9,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1110 Cell Name   UCB408E6E7TERT-33
LOT No.   07202005 Lot Specification   distribution
Medium   PLUSOID-M(complete medium) Temperature   37 C
Cell Density at Seeding   3.3x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.2x10^5
Viability at cell freezing (%)   95.1 Antibiotics Used   NT
Passage Number   P27 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LD examined. Human, not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
D13S317:9,11
D7S820:8,10
D16S539:9,10
VWA:17,18
TH01:7,9
AM:X,Y
TPOX:9,11
CSF1PO:11
Adhesion   Yes Exoteric Gene   Nt
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
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