JCRB1112 Yub621b

Cell information

Important Notice(s)

POWEREDBY10 medium

不死化していないヒト体性幹細胞の分譲について(倫理的な取り扱い)

On the distribution of human non-immortalized somatic stem cells to oversea users (ethical ragulation).

Cell type:primary mesenchymal stem cell (View Pricing Information)

JCRB No.   JCRB1112 Cell Name   Yub621b
Profile   from polydactylous finger Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   mesenchymal stem cell
Case history    Metastasis   No
Tissue Metastasized    Genetics   -
Life Span   finite Crisis PDL   20PD
Morphology   fibroblast-like Character   -
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2005
Medium   poweredby10 Methods for Passages   -
Cell Number on Passage   1 x 10^5 cells/10cm dish Race   Japanese
CO2 Conc.   5% Tissue Sampling   polydactylous human fingers (finger
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3258
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LOT Information

Cell No.   JCRB1112 Cell Name   Yub621b
LOT No.   07102008 Lot Specification   distribution
Medium   POWEREDBY10 (HIDA Cat#6012) Temperature   37 C
Cell Density at Seeding   4.6x10^3 cells/sq.cm Methods for Passages   Cells were harvested treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   96.8 Antibiotics Used   NT
Passage Number   p8 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8,11
D7S820:11
D16S539:11,12
VWA:17,18
TH01:9
AM:X
TPOX:8,11
CSF1PO:10,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1112 Cell Name   Yub621b
LOT No.   07212005 Lot Specification   distribution
Medium   POWEREDBY10 Temperature   37 C
Cell Density at Seeding   2.9x10^3 cells/sq.cm Methods for Passages   Cells were harvested treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.2x10^5
Viability at cell freezing (%)   86.4 Antibiotics Used   Free
Passage Number   P7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8,11
D7S820:11
D16S539:11,12
VWA:17,18
TH01:9
AM:X
TPOX:8,11
CSF1PO:10,14
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
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