JCRB0257 CoCM-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0257 Cell Name   CoCM-1
Profile   Human tumor cell line of the moderately differentiated adenocarcinoma from descending colon. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   45
Identity   available Tissue for Primary Cancer   colon
Case history    Metastasis   
Tissue Metastasized    Genetics   descending colon, adenocarcinoma
Life Span   infinite Crisis PDL   
Morphology   cluster formation Character   Moderately differentiated adenocarcinoma from descending colon.
Classify   tumor Established by   Kataoka,H.
Registered by   Kataoka, H. Regulation for Distribution   Please send a reprint to Kataoka, H.
Comment   Moderately differentiated. Year   1989
Medium   45% RPMI1640 medium with 45% Ham's F12 medium and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.125% trypsin.
Cell Number on Passage   split ratio=1/4,every 2-3 wks Race   
CO2 Conc.   5 % Tissue Sampling   descending colon
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1127
Reference
Pubmed id:3198327A new long-term in vitro invasion assay using fibrous connective tissue matrices maintaining architectural characteristics of connective tissue.
Nabeshima K,Kataoka H,Koita H,Murayama T,Koono M
Invasion Metastasis. 1988;8(5):301-16

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LOT Information

Cell No.   JCRB0257 Cell Name   
LOT No.   12222011 Lot Specification   distribution
Medium   1:1 mixture of RPMI 1640 medium and Ham's F12 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA.. Passage twice a week to avoid the tight adhesion to culture dish. This cell line forms islands of tightly packed cells, and does not reach confluent.
Doubling Time   approx. 59 hrs. Cell Number in Vial (cells/1ml)   4.8 x 10^5
Viability at cell freezing (%)   82.8 Antibiotics Used   free
Passage Number   P81 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DSMO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0257 Cell Name   
LOT No.   06302006 Lot Specification   distribution
Medium   1:1 mixture of RPMI 1640 medium and Ham's F12 medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1 - 8 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. This cell line forms islands of tightly packed cells, and does not reach confluent.
Doubling Time   approx. 34 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   P176 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0257 Cell Name   
LOT No.   031395 Lot Specification   distribution
Medium   RPMI 1640 + Ham's F12 (1:1) + FCS 10% Temperature   37
Cell Density at Seeding   1/4 per 2-3 weeks Methods for Passages   0.02% EDTA and 0.125% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   91.6 Antibiotics Used   free
Passage Number   P72 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST, MPI, MD, PEPB, G6PD, LD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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