JCRB0259 MSK

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0259 Cell Name   MSK
Profile   osteosarcoma, 32P-induced, (F344 rat) Other Name   MSK
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   sarcoma
Case history    Metastasis   
Tissue Metastasized    Genetics   32P-induced osteosarcoma, F344 rat, female
Life Span   infinite Crisis PDL   
Morphology    Character   32P-induced osteosarcoma, F344 rat, female
Classify    Established by   Takahashi,K.
Registered by   Sato, K. Regulation for Distribution   Contact to K. Sato at distribution of cells.
Comment   High tumorgenecity and metastasis in a F344 rat. Year   1989
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.1% trypsin.
Cell Number on Passage   10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   osteosarcoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8369
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LOT Information

Cell No.   JCRB0259 Cell Name   MSK
LOT No.   051695 Lot Specification   FDSC/distribution
Medium   dulbecco's modified Eagle's medium with 10% fetal bovine serum (Bio cell, 4001776) Temperature   37 C
Cell Density at Seeding   1.6x10^5 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.6x10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   P186 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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