JCRB0260 SAS

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0260 Cell Name   SAS
Profile   squamous carcinoma, tongue Other Name   SAS
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   oral
Case history    Metastasis   
Tissue Metastasized    Genetics   tongue, squamous carcinoma
Life Span    Crisis PDL   
Morphology    Character   tongue, squamous carcinoma
Classify    Established by   Takahashi,K.
Registered by   Sato, K. Regulation for Distribution   Contact to K. Sato at distribution of cells.
Comment   Derived from primary focus. Year   1989
Medium   45% Dulbecco's modified Eagle's medium with 45% Ham's F12 medium and 10% fetal calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.1% trypsin.
Cell Number on Passage   10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   tongue
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1675
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909708
Reference
Pubmed id:noneEstablishment and characterization of a cell line (SAS) from poorly defferentiated human squamous cell carcinoma of the tongue.
Takahashi,K., Kanazawa,H., Akiyama,Y., Tazaki,S., Takahara,M., Muto,T., Tanzawa,H., and Sato,K.
J. Jpn. Stomatol. Soc., 1989, 38(1): 20-28.

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LOT Information

Cell No.   JCRB0260 Cell Name   SAS
LOT No.   07312007 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS lot; GIBCO 484375) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.1% trypsin and 0.02% EDTA
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   5.6 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   P124 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   96% Additional information   
Cell No.   JCRB0260 Cell Name   SAS
LOT No.   052695 Lot Specification   FDSC/distribution
Medium   Dulbecco's modified Eagle medium 50% with Ham's F12 medium 50% and 10% fetal bovine serum (JRH12103) Temperature   37 C
Cell Density at Seeding   6.44x10^4 cells/ml Methods for Passages   Treat cells with 0.25% trypsin and 0.02%.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.46x10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   P120 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0260 Cell Name   SAS
LOT No.   06212013 Lot Specification   distribution
Medium   45% Dulbecco's modified Eagle's medium with 45% Ham's F12 medium and 10% fetal calf serum (FBS: GIBCO Cat. No. 10099) Temperature   37C
Cell Density at Seeding   approx. 10^5 cells/mL Methods for Passages   Cells are treated with 0.02% EDTA and 0.1% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   2.8 x 10^6 cells/1mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P124 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)   87.4 Additional information   
Cell No.   JCRB0260 Cell Name   SAS
LOT No.   08022016 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   4.4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 21 hrs. Cell Number in Vial (cells/1ml)   5.7 x 10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   P123 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9
D13S317:10,12
D7S820:11,12
D16S539:12,13
VWA:14,17
TH01:6,7
AM:X
TPOX:11,12
CSF1PO:11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
Cell No.   JCRB0260 Cell Name   SAS
LOT No.   01192021 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin solution.
Doubling Time   21 - 34 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   P123 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   97 Additional information   
Cell No.   JCRB0260 Cell Name   SAS
LOT No.   01292024 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F-12 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   2.9 - 5.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   40 - 51 hrs. Cell Number in Vial (cells/1ml)   6.2 x 10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   P124 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9
D13S317:10,12
D7S820:11,12
D16S539:12,13
VWA:14,17
TH01:6,7
AM:X
TPOX:11,12
CSF1PO:11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - DMEM/F-12 CO2 Conc.   5%
Viability immediately after thawing (%)   95.9 Additional information   
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