JCRB1117 HEC-1-A
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1117 | Cell Name | HEC-1-A |
|---|---|---|---|
| Profile | Human endometroid adenocarcinoma, G2 from Japanese female. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | uterus |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | endometrial carcinoma | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | endometrial carcinoma |
| Classify | tumor | Established by | Kuramoto, H. |
| Registered by | Kuramoto, H. | Regulation for Distribution | |
| Comment | Year | 2005 | |
| Medium | TC199 medium with 10% calf serum (the information at the establishment of this cell line. Currently, the medium for propagation is Eagle's minimum essential medium with 15% FBS). | Methods for Passages | Harvested with 0.1% trypsin and 0.02% EDTA. |
| Cell Number on Passage | 1-2 x 10^5 cells/mL | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | uterine corpus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:15053063 | Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines. Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H Eur J Gynaecol Oncol. 2004;25(1):55-60 |
| Pubmed id:269609 | Cytogenetic studies of human endometrial carcinomas by means of tissue culture. Kuramoto H,Hamano M Acta Cytol. 1977 Jul-Aug;21(4):559-65 |
| Pubmed id:4673779 | Establishment of a cell line of human endometrial adenocarcinoma in vitro. Kuramoto H,Tamura S,Notake Y Am J Obstet Gynecol. 1972 Dec 15;114(8):1012-9 |
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LOT Information
| Cell No. | JCRB1117 | Cell Name | HEC-1-A |
|---|---|---|---|
| LOT No. | 10292010 | Lot Specification | distribution |
| Medium | MEM(GIBCO) with 15% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 2.0-8.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.53x10^6 |
| Viability at cell freezing (%) | 94.9 | Antibiotics Used | free |
| Passage Number | p790 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12,15,16 D13S317:11,14 D7S820:9,11 D16S539:12,13 VWA:19,23 TH01:6,7 AM:X TPOX:8,11 CSF1PO:10,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1117 | Cell Name | |
|---|---|---|---|
| LOT No. | 08032010 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 15% fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 7.7 - 8.2 x 10^4 cells/mL | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 54 hrs | Cell Number in Vial (cells/1ml) | 4.4 x 10^6 |
| Viability at cell freezing (%) | 87.8 | Antibiotics Used | free |
| Passage Number | P787 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1117 | Cell Name | HEC-1-A |
|---|---|---|---|
| LOT No. | 08182005 | Lot Specification | distribution |
| Medium | EMEM with 15% heat inactivated fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 2.2x10^4 cells/sq.cm | Methods for Passages | Cells were harvested treatment with 0.25% trypsin and 0.02% EDTA |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.4x10^6 |
| Viability at cell freezing (%) | 87.2 | Antibiotics Used | Free |
| Passage Number | P786 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,15 D13S317:11 D7S820:9,11 D16S539:12,13 VWA:19,20,23,24 TH01:6,7 AM:X TPOX:8,9,11 CSF1PO:10,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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