JCRB1117 HEC-1-A

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1117 Cell Name   HEC-1-A
Profile   Human endometroid adenocarcinoma, G2 from Japanese female. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   No
Tissue Metastasized    Genetics   endometrial carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endometrial carcinoma
Classify   tumor Established by   Kuramoto, H.
Registered by   Kuramoto, H. Regulation for Distribution   
Comment    Year   2005
Medium   TC199 medium with 10% calf serum (the information at the establishment of this cell line. Currently, the medium for propagation is Eagle's minimum essential medium with 15% FBS). Methods for Passages   Harvested with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1-2 x 10^5 cells/mL Race   Japanese
CO2 Conc.   5 % Tissue Sampling   uterine corpus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0293
Reference
Pubmed id:15053063 Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60
Pubmed id:269609 Cytogenetic studies of human endometrial carcinomas by means of tissue culture.
Kuramoto H,Hamano M
Acta Cytol. 1977 Jul-Aug;21(4):559-65
Pubmed id:4673779 Establishment of a cell line of human endometrial adenocarcinoma in vitro.
Kuramoto H,Tamura S,Notake Y
Am J Obstet Gynecol. 1972 Dec 15;114(8):1012-9

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LOT Information

Cell No.   JCRB1117 Cell Name   HEC-1-A
LOT No.   10292010 Lot Specification   distribution
Medium   MEM(GIBCO) with 15% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   2.0-8.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.53x10^6
Viability at cell freezing (%)   94.9 Antibiotics Used   free
Passage Number   p790 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12,15,16
D13S317:11,14
D7S820:9,11
D16S539:12,13
VWA:19,23
TH01:6,7
AM:X
TPOX:8,11
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1117 Cell Name   
LOT No.   08032010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   7.7 - 8.2 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 54 hrs Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability at cell freezing (%)   87.8 Antibiotics Used   free
Passage Number   P787 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1117 Cell Name   HEC-1-A
LOT No.   08182005 Lot Specification   distribution
Medium   EMEM with 15% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   2.2x10^4 cells/sq.cm Methods for Passages   Cells were harvested treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   87.2 Antibiotics Used   Free
Passage Number   P786 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,15
D13S317:11
D7S820:9,11
D16S539:12,13
VWA:19,20,23,24
TH01:6,7
AM:X
TPOX:8,9,11
CSF1PO:10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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