JCRB1118 HEC-6
Cell information
Cell type:general cells (View Pricing Information)
JCRB No. | JCRB1118 | Cell Name | HEC-6 |
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Profile | Human endometrial adenocarcinoma cell line. | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | F | Age | |
Identity | available | Tissue for Primary Cancer | uterus |
Case history | Metastasis | No | |
Tissue Metastasized | Genetics | endometrial adenoacanthoma | |
Life Span | infinite | Crisis PDL | |
Morphology | epithelial-like | Character | endometrial adenocarcinoma |
Classify | tumor | Established by | Hamano, M. & Kuramoto, H. |
Registered by | Kuramoto, H. | Regulation for Distribution | |
Comment | Year | 2005 | |
Medium | Eagle's minimal essential medium with 15% fetal calf serum. | Methods for Passages | Harvested with 0.1% trypsin and 0.02% EDTA. |
Cell Number on Passage | 1-2 10^5 cells/ml | Race | Japanese |
CO2 Conc. | 5 % | Tissue Sampling | uterine body |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
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Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Reference | |
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Pubmed id:15053063 | Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines. Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H Eur J Gynaecol Oncol. 2004;25(1):55-60 |
Pubmed id:862644 | Establishment and characterization of the cell-line of a human edometrial adenoacanthoma. Kuramoto H,Hamano M Eur J Cancer. 1977 Mar 29;13(3):253-9 |
Pubmed id:269609 | Cytogenetic studies of human endometrial carcinomas by means of tissue culture. Kuramoto H,Hamano M Acta Cytol. 1977 Jul-Aug;21(4):559-65 |
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LOT Information
Cell No. | JCRB1118 | Cell Name | |
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LOT No. | 10112012 | Lot Specification | distribution |
Medium | Eagle's minimum essential medium with 15% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10099) | Temperature | 37C |
Cell Density at Seeding | 3.5 - 7.2 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. |
Doubling Time | approx. 43 hrs | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
Viability at cell freezing (%) | 88.2 | Antibiotics Used | free |
Passage Number | P772 | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
Viability immediately after thawing (%) | Additional information |
Cell No. | JCRB1118 | Cell Name | HEC-6 |
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LOT No. | 08172005 | Lot Specification | distribution |
Medium | Eagle's minimal essential medium with 15% heat inactivated fetal bovine serum | Temperature | 37 C |
Cell Density at Seeding | 2.8x10^4 | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA |
Doubling Time | Nt | Cell Number in Vial (cells/1ml) | 1.5x10^6 |
Viability at cell freezing (%) | 93.4 | Antibiotics Used | Free |
Passage Number | P769 | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | G6PD(typeB), NP, LD examined. Human, not HeLa |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | D5S818:13,14,15 D13S317:11,12 D7S820:8,9,11,12 D16S539:8,10 VWA:16,18,19 TH01:6,9,10 AM:X TPOX:8,9,10 CSF1PO:9,10,13 |
Adhesion | Yes | Exoteric Gene | NT |
Medium for Freezing | Culture medium with 5% DMSO | CO2 Conc. | 5 % |
Viability immediately after thawing (%) | 88% | Additional information |
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Cell No. | JCRB1118 | Cell Name | HEC-6 |
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LOT No. | 07182018 | Lot Specification | distribution |
Medium | Eagle's minimum essential medium with 15% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
Cell Density at Seeding | 3 - 4 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. |
Doubling Time | approx. 1.5 days. | Cell Number in Vial (cells/1ml) | 1.8 x 10^6 |
Viability at cell freezing (%) | 86 | Antibiotics Used | free |
Passage Number | P772 | PDL | |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | Chromosome Information | ||
Surface Antigen | DNA Profile (STR) | ||
Adhesion | Yes | Exoteric Gene | |
Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 86 | Additional information |
Cell No. | JCRB1118 | Cell Name | HEC-6 |
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LOT No. | 04192023 | Lot Specification | distribution |
Medium | MEM (GIBCO cat.No.11095-080) with 15% heat inactivated fetal bovine serum (SIGMA cat.No.172012 LotNo.12J396) | Temperature | 37 C |
Cell Density at Seeding | 1.34-3.63x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin (GIBCO) and 0.02% EDTA. |
Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.3x10^7 |
Viability at cell freezing (%) | 98.93 | Antibiotics Used | free |
Passage Number | p774 | PDL | NT |
Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
Sterility: FUNGI | - | Isozyme Analysis | |
Chromosome Mode | NT | Chromosome Information | NT |
Surface Antigen | NT | DNA Profile (STR) | D5S818:13,14,15 D13S317:11,12 D7S820:8,9,11,12 D16S539:8,10 VWA:16,18 TH01:6,9,10 AM:X TPOX:8,9,10 CSF1PO:9,10,13 |
Adhesion | Yes | Exoteric Gene | NT |
Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
Viability immediately after thawing (%) | 93.78 | Additional information |
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