JCRB1118 HEC-6

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1118 Cell Name   HEC-6
Profile   Human endometrial adenocarcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   No
Tissue Metastasized    Genetics   endometrial adenoacanthoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endometrial adenocarcinoma
Classify   tumor Established by   Hamano, M. & Kuramoto, H.
Registered by   Kuramoto, H. Regulation for Distribution   
Comment    Year   2005
Medium   Eagle's minimal essential medium with 15% fetal calf serum. Methods for Passages   Harvested with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1-2 10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   uterine body
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2931
Reference
Pubmed id:15053063Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60
Pubmed id:862644Establishment and characterization of the cell-line of a human edometrial adenoacanthoma.
Kuramoto H,Hamano M
Eur J Cancer. 1977 Mar 29;13(3):253-9
Pubmed id:269609Cytogenetic studies of human endometrial carcinomas by means of tissue culture.
Kuramoto H,Hamano M
Acta Cytol. 1977 Jul-Aug;21(4):559-65

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LOT Information

Cell No.   JCRB1118 Cell Name   
LOT No.   10112012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37C
Cell Density at Seeding   3.5 - 7.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 43 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   88.2 Antibiotics Used   free
Passage Number   P772 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1118 Cell Name   HEC-6
LOT No.   08172005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 15% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   2.8x10^4 Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   Nt Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   93.4 Antibiotics Used   Free
Passage Number   P769 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LD examined. Human, not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13,14,15
D13S317:11,12
D7S820:8,9,11,12
D16S539:8,10
VWA:16,18,19
TH01:6,9,10
AM:X
TPOX:8,9,10
CSF1PO:9,10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)   88% Additional information   
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Cell No.   JCRB1118 Cell Name   HEC-6
LOT No.   07182018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 days. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   86 Antibiotics Used   free
Passage Number   P772 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   86 Additional information   
Cell No.   JCRB1118 Cell Name   HEC-6
LOT No.   04192023 Lot Specification   distribution
Medium   MEM (GIBCO cat.No.11095-080) with 15% heat inactivated fetal bovine serum (SIGMA cat.No.172012 LotNo.12J396) Temperature   37 C
Cell Density at Seeding   1.34-3.63x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin (GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^7
Viability at cell freezing (%)   98.93 Antibiotics Used   free
Passage Number   p774 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13,14,15
D13S317:11,12
D7S820:8,9,11,12
D16S539:8,10
VWA:16,18
TH01:6,9,10
AM:X
TPOX:8,9,10
CSF1PO:9,10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   93.78 Additional information   
Images
Cell No.   JCRB1118 Cell Name   HEC-6
LOT No.   08092024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   2.6 - 3.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 54 hrs. Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   91 Antibiotics Used   free
Passage Number   P772 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
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