JCRB0305 XP2SA

Cell information

Important Notice(s)

XP(色素性乾皮症患者)由来細胞

Cell type:human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB0305 Cell Name   XP2SA
Profile   xeroderma pigmentosum (variant type), fibroblast Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   44
Identity   available Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   karyotype=46,XX
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   Xeroderma pigmentosum (Variant), fibroblast. Doubling time=24hr.
Classify    Established by   Ikenaga,M.
Registered by   Ikenaga,M. Regulation for Distribution   
Comment   Hereditary disease with high cancer risk,xeroderma pigmentosum (autosomal recessive), skin fibroblast. sister of XP1SA Year   1985
Medium   RPMI1640 medium, 20% non-heat-inactivated fetal bovine serum. (establish: Dulbecco's modified Eagle's medium with 10% fetal calf serum.) Methods for Passages   trypsin
Cell Number on Passage   split ratio=1/3 Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 + HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3243
Reference
Pubmed id:3814505 Sensitivity to UV radiation of fibroblasts from a Japanese group A xeroderma pigmentosum patient with mild neurological abnormalities.
Sato K,Watatani M,Ikenaga M,Kozuka T,Kitano Y,Yoshikawa K,Mimaki T,Abe J,Sugita T
Br J Dermatol. 1987 Jan;116(1):101-8
Pubmed id:7155047 Kinetic analysis of polyethylene glycol-induced cell fusion in cultured human fibroblasts: its application to genetic complementation analysis of xeroderma pigmentosum.
Sato K,Ikenaga M,Sano S
Med J Osaka Univ. 1982 Sep;33(1-2):19-28

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LOT Information

Cell No.   JCRB0305 Cell Name   XP2SA
LOT No.   03182011 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA027K03911,non HI) Temperature   37 C
Cell Density at Seeding   9.71-13.3x10^3cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.04x10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   p9 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8,9
D7S820:11
D16S539:9
VWA:18
TH01:6
AM:X
TPOX:11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB0305 Cell Name   XP2SA
LOT No.   02102017 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% fetal bovine serum(SIGMA12J396,non HI) Temperature   37 C
Cell Density at Seeding   1.2-2.1x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   p13 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8,9
D7S820:11
D16S539:9
VWA:18
TH01:6
AM:X
TPOX:11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
Images
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