JCRB1123 HEC-108

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1123 Cell Name   HEC-108
Profile   Human tumor cell line from uterine endometrium. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endometrial adenocarcinoma
Classify   tumor Established by   Kuramoto,H.
Registered by   Kuramoto, H. Regulation for Distribution   
Comment    Year   2005
Medium   minimal essential medium with 15% fetal calf serum Methods for Passages   0.1 % trypsin and 0.02 % EDTA
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   uterine corpus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2923
Reference
Pubmed id:15053063Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60
Pubmed id:2064198Establishment and characterization of human endometrial cancer cell lines.
Kuramoto H,Nishida M,Morisawa T,Hamano M,Hata H,Kato Y,Ohno E,Iida T
Ann N Y Acad Sci. 1991;622():402-21
Pubmed id:2979208[Establishment and characterization of a new SCC antigen producing cell line (HCS-2) from a carcinoma of the uterine cervix].
Morisawa T,Kuramoto H,Shimoda T,Sakamoto I,Kato Y,Hamano M
Hum Cell. 1988 Sep;1(3):308-14
Pubmed id:269609Cytogenetic studies of human endometrial carcinomas by means of tissue culture.
Kuramoto H,Hamano M
Acta Cytol. 1977 Jul-Aug;21(4):559-65

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LOT Information

Cell No.   JCRB1123 Cell Name   HEC-108
LOT No.   07072012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   6.3 - 6.9 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   76.4 Antibiotics Used   free
Passage Number   P316 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   Lot# is mislabeled as 07072011.
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Cell No.   JCRB1123 Cell Name   HEC-108
LOT No.   09302010 Lot Specification   distribution
Medium   Minimum essential medium with 15% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.2-1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.58x10^6
Viability at cell freezing (%)   94.8 Antibiotics Used   free
Passage Number   p317 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:6,12
D13S317:9,10,11,13,14
D7S820:8,9,12
D16S539:12,13
VWA:15,16,17
TH01:8,9
AM:X
TPOX:11,12
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1123 Cell Name   HEC-108
LOT No.   09062005 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   3.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   87.2 Antibiotics Used   Free
Passage Number   p305 PDL   NT
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1123 Cell Name   HEC-108
LOT No.   11242005 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   2.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   MC210 to remove mycoplasmas
Passage Number   P315 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:6,12
D13S317:9,10,11,13,14
D7S820:8,9,12
D16S539:12,13
VWA:15,16,17
TH01:8,9
AM:X
TPOX:11,12
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1123 Cell Name   HEC-108
LOT No.   04222022 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-080) with 15% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   2.1-3.6x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   98.10 Antibiotics Used   free
Passage Number   p316 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:6,12
D13S317:9,10,11,13,14
D7S820:8,9,12
D16S539:12,13
VWA:15,16,17
TH01:8,9
AM:X
TPOX:11,12
CSF1PO:11,12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   85.9 Additional information   
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