JCRB1125 PL502

Cell information

Important Notice(s)

PLUSOID-M medium

Mycoplasmal contamination of JCRB1125 PL502

不死化していないヒト体性幹細胞の分譲について(倫理的な取り扱い)

On the distribution of human non-immortalized somatic stem cells to oversea users (ethical ragulation).

Cell type:primary mesenchymal stem cell (View Pricing Information)

JCRB No.   JCRB1125 Cell Name   PL502
Profile   Human placenta-derived mesenchymal stem cells Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   mesenchymal stem cell
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2005
Medium   *** Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-quarter of harvested cells.
Cell Number on Passage   1 x 10^5/10 cm dish Race   
CO2 Conc.   5% Tissue Sampling   placenta
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3126
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LOT Information

Cell No.   JCRB1125 Cell Name   PL502
LOT No.   09222005 Lot Specification   distribution
Medium   PLUSOID-M(complete medium) Temperature   37 C
Cell Density at Seeding   1.9x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.1x10^5
Viability at cell freezing (%)   97.7 Antibiotics Used   
Passage Number   p8 PDL   NT
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   46 Chromosome Information   45/1 46/49 >90/2 (52)
Surface Antigen   NT DNA Profile (STR)   D5S818:9,11,13
D13S317:8,10,12
D7S820:9,10,11
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   Nt
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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