JCRB1122 HEC-151

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1122 Cell Name   HEC-151
Profile   Human endometrioid adenocarcinoma, G2. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   No
Tissue Metastasized    Genetics   endometrial carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endometrial carcinoma
Classify   tumor Established by   Hamano,M. & Kuramoto, H.
Registered by   Kuramoto, H. Regulation for Distribution   
Comment    Year   2005
Medium   Eagle's minimal essential medium with 15% fetal calf serum. Methods for Passages   Harvested with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1-2 x 10^5 cells/mL Race   Japanese
CO2 Conc.   5 % Tissue Sampling   uterine corpus
Virus Summary   tested Virus Tested   CMV(-), EBV(-), HHV6(-), HHV7(-), BKV(-), JCV(-), ADV(-), parvoB19(-), HBV(-), HTLV1(-), HTLV2(-), HIV1(-), HIV2(-), HPV18(-) [-/negative, +/positive]
Tissue Type   
Reference
Pubmed id:15053063 Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60

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LOT Information

Cell No.   JCRB1122 Cell Name   HEC-151
LOT No.   08242010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.03-1.69x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.44x10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   p333 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,14
D13S317:8,13,14
D7S820:8,11
D16S539:9,11
VWA:14,19
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:7,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1122 Cell Name   HEC-151
LOT No.   03312006 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   9.0x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.9x10^6
Viability at cell freezing (%)   90.1 Antibiotics Used   Free
Passage Number   P333 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13,14
D13S317:8,13,14
D7S820:8,11
D16S539:9,11
VWA:14,19
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:7,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1122 Cell Name   HEC-151
LOT No.   08312005 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time   NT Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   + Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1122 Cell Name   HEC-151
LOT No.   10302014 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.4 - 2.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 19 hrs Cell Number in Vial (cells/1ml)   6.0 x 10^6
Viability at cell freezing (%)   73.6 Antibiotics Used   free
Passage Number   P334 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   73.6
Additional information   
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