JCRB1122 HEC-151
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1122 | Cell Name | HEC-151 |
|---|---|---|---|
| Profile | Human endometrioid adenocarcinoma, G2. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | uterus |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | endometrial carcinoma | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | endometrial carcinoma |
| Classify | tumor | Established by | Hamano,M. & Kuramoto, H. |
| Registered by | Kuramoto, H. | Regulation for Distribution | |
| Comment | Year | 2005 | |
| Medium | Eagle's minimal essential medium with 15% fetal calf serum. | Methods for Passages | Harvested with 0.1% trypsin and 0.02% EDTA. |
| Cell Number on Passage | 1-2 x 10^5 cells/mL | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | uterine corpus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:15053063 | Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines. Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H Eur J Gynaecol Oncol. 2004;25(1):55-60 |
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LOT Information
| Cell No. | JCRB1122 | Cell Name | HEC-151 |
|---|---|---|---|
| LOT No. | 08242010 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum(SIGMA Cat. #F0926 Lot 027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 1.03-1.69x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.44x10^6 |
| Viability at cell freezing (%) | 94.0 | Antibiotics Used | free |
| Passage Number | p333 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,14 D13S317:8,13,14 D7S820:8,11 D16S539:9,11 VWA:14,19 TH01:6,9 AM:X TPOX:8,11 CSF1PO:7,11 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1122 | Cell Name | HEC-151 |
|---|---|---|---|
| LOT No. | 03312006 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 9.0x10^3 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.9x10^6 |
| Viability at cell freezing (%) | 90.1 | Antibiotics Used | Free |
| Passage Number | P333 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,13,14 D13S317:8,13,14 D7S820:8,11 D16S539:9,11 VWA:14,19 TH01:6,9 AM:X TPOX:8,11 CSF1PO:7,11 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1122 | Cell Name | HEC-151 |
|---|---|---|---|
| LOT No. | 08312005 | Lot Specification | distribution |
| Medium | Temperature | ||
| Cell Density at Seeding | Methods for Passages | ||
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | |
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | ||
| Sterility: MYCOPLASMA | + | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1122 | Cell Name | HEC-151 |
|---|---|---|---|
| LOT No. | 10302014 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 1.4 - 2.3 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 19 hrs | Cell Number in Vial (cells/1ml) | 6.0 x 10^6 |
| Viability at cell freezing (%) | 73.6 | Antibiotics Used | free |
| Passage Number | P334 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 73.6 | Additional information |