JCRB1131 UE6E7T-1

Cell information

Important Notice(s)

POWEREDBY10 medium

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1131 Cell Name   UE6E7T-1
Profile   Human bone marrow-derived mesenchymal stem cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   91
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Human bone marrow-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the E6, E7, and hTERT.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2005
Medium   Poweredby10 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   bone marrow

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8306
Pubmed id:15923633Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mori T,Kiyono T,Imabayashi H,Takeda Y,Tsuchiya K,Miyoshi S,Makino H,Matsumoto K,Saito H,Ogawa S,Sakamoto M,Hata J,Umezawa A
Mol Cell Biol. 2005 Jun;25(12):5183-95
Pubmed id:15293342Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?
Takeda Y,Mori T,Imabayashi H,Kiyono T,Gojo S,Miyoshi S,Hida N,Ita M,Segawa K,Ogawa S,Sakamoto M,Nakamura S,Umezawa A
J Gene Med. 2004 Aug;6(8):833-45


LOT Information

Cell No.   JCRB1131 Cell Name   UE6E7T-1
LOT No.   11012005 Lot Specification   distribution
Medium    Temperature   37 C
Cell Density at Seeding    Methods for Passages   
Doubling Time   NT Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   Free
Passage Number    PDL   NT
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
Adhesion    Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information