JCRB1136 UE6E7T-3

Cell information

Important Notice(s)

POWEREDBY10 medium

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1136 Cell Name   UE6E7T-3
Profile   Human bone marrow-derived mesenchymal stem cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   91
Identity   available Tissue for Primary Cancer   mesenchymal stem cell
Case history    Metastasis   No
Tissue Metastasized    Genetics   Human bone marrow-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the E6, E7, and hTERT.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2005
Medium   Poweredby10 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   bone marrow
Tissue Type   bone marrow

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3237
Reference
Pubmed id:15923633 Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential.
Mori T,Kiyono T,Imabayashi H,Takeda Y,Tsuchiya K,Miyoshi S,Makino H,Matsumoto K,Saito H,Ogawa S,Sakamoto M,Hata J,Umezawa A
Mol Cell Biol. 2005 Jun;25(12):5183-95
Pubmed id:15293342 Can the life span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation?
Takeda Y,Mori T,Imabayashi H,Kiyono T,Gojo S,Miyoshi S,Hida N,Ita M,Segawa K,Ogawa S,Sakamoto M,Nakamura S,Umezawa A
J Gene Med. 2004 Aug;6(8):833-45

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LOT Information

Cell No.   JCRB1136 Cell Name   
LOT No.   01192009 Lot Specification   distribution
Medium   POWERDBY10 medium (Med Shirotori, Co. Cat. #6012) Temperature   37 C
Cell Density at Seeding   approx. 2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 1 mM EDTA. Do not arrow to be full confluency.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   78.9 Antibiotics Used   not added
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Cell Banker BLC-1 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1136 Cell Name   UE6E7T-3
LOT No.   11302005 Lot Specification   distribution
Medium   POWEREDBY10 Temperature   37 C
Cell Density at Seeding   2.0x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.9x10^5
Viability at cell freezing (%)   98.2 Antibiotics Used   Free
Passage Number   p23 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:8
D7S820:8,11
D16S539:11,12
VWA:17,18
TH01:9
AM:X
TPOX:9,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Images
Cell No.   JCRB1136 Cell Name   UE6E7T-3
LOT No.   06182018 Lot Specification   distribution
Medium   Poweredby10 (GlycoTechnica, Ltd.) Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^4 cells/mL Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA.
Doubling Time   Slow Cell Number in Vial (cells/1ml)   8.0 x 10^5
Viability at cell freezing (%)   91.8 Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   91.8
Additional information   
Cell No.   JCRB1136 Cell Name   UE6E7T-3
LOT No.   06172020 Lot Specification   distribution
Medium   POWEREDBY10(within serum) catalog No.6012 Temperature   37 C
Cell Density at Seeding   0.6-1.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.(1/5 split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   99.10 Antibiotics Used   
Passage Number   p24 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:8
D7S820:8,11
D16S539:11,12
VWA:17,18
TH01:9
AM:X
TPOX:9,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   93.7
Additional information   
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