JCRB0810 ME-180
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0810 | Cell Name | ME-180 |
|---|---|---|---|
| Profile | cervical carcinoama | Other Name | ME-180 |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 66 |
| Identity | available | Tissue for Primary Cancer | uterine cervix |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | uterus, cervical cancer | |
| Life Span | Crisis PDL | ||
| Morphology | Character | uterus, cervical cancer | |
| Classify | Established by | Sykes,J. | |
| Registered by | Akiyama,M. | Regulation for Distribution | |
| Comment | Year | 1986 | |
| Medium | Eagle's minimal essential medium with 10% fetal calf serum. | Methods for Passages | Treat cells with 0.25% trypsin and dilute in fresh medium. Change medium twice a week. |
| Cell Number on Passage | 2.2 x 10^4 cells/sq.cm. | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | cervix |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
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LOT Information
| Cell No. | JCRB0810 | Cell Name | ME-180 |
|---|---|---|---|
| LOT No. | 090498 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (Summit 8.1E+41). | Temperature | 37 C |
| Cell Density at Seeding | 2.2 x 10^4 cells/sq.cm. | Methods for Passages | Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.0 x 10^6 |
| Viability at cell freezing (%) | 97.0 | Antibiotics Used | free |
| Passage Number | P30 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD, LD, NP examained. Species confirmed. |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:11,13 D7S820:9,10 D16S539:12,13 VWA:15,16,17 TH01:8,10 AM:X TPOX:8,10 CSF1PO:11 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO. | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB0810 | Cell Name | |
|---|---|---|---|
| LOT No. | 090595 | Lot Specification | distribution |
| Medium | MEM + 10% FCS | Temperature | 37 |
| Cell Density at Seeding | Methods for Passages | 0.25% trypsin and dilute in fresh medium. Change medium twice a week. | |
| Doubling Time | Cell Number in Vial (cells/1ml) | ca3-5x10^6 | |
| Viability at cell freezing (%) | Antibiotics Used | penicillin/streptmycin | |
| Passage Number | P25 (BM3) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | confirmed as human |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |