-Detailed Information [JCRB1142]-

JCRB1142 HEC-265

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB1142	Cell Name	HEC-265
Profile	Human endometrioid adenocarcinoma, G1 from uterus.	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age
Identity	available	Tissue for Primary Cancer	uterus
Case history		Metastasis	No
Tissue Metastasized		Genetics	endometrial carcinoma
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	endometrial carcinoma
Classify	tumor	Established by	Fujisawa,T. & Kuramoto, H.
Registered by	Kuramoto, H.	Regulation for Distribution	free
Comment		Year	2005
Medium	Eagle's minimal essential medium with 15% fetal calf serum.	Methods for Passages	Cells were harvested after treatment with 0.1% trypisn and 0.02% EDTA.
Cell Number on Passage	1-2 x10^5 cells/mL	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	uterine corpus
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2928

Reference
Pubmed id:15171304	Establishment and characterization of two different types of new human endometrial adenocarcinoma cell lines (HEC-251 and HEC-265). Fujisawa T,Hamano M,Hata H,Kamata Y,Watanabe J,Sekimoto R,Kuramoto H Eur J Gynaecol Oncol. 2004;25(3):299-304
Pubmed id:15053063	Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines. Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H Eur J Gynaecol Oncol. 2004;25(1):55-60

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Pubmed id:none	ヒト子宮類内膜がん細胞株表面における免疫チェックポイント分子PD-L1の発現調節に果たすEzrin/Radixin/Moesinの役割小堀宅郎, 田中千都, 伊藤優衣, 浦嶋庸子, 伊藤卓也, 高垣伸匡, 小畑友紀雄 Journal of AYA Oncology Alliance　Vol.3 No.1 2023

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LOT Information

02072006
06132013
12152017
08062020
09192023

Cell No.	JCRB1142	Cell Name	HEC-265
LOT No.	02072006	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum	Temperature	37 C
Cell Density at Seeding	2.9x10^3 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time	NT	Cell Number in Vial (cells/1ml)	4.7x10^6
Viability at cell freezing (%)	91.4	Antibiotics Used	Free
Passage Number	P187	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP, LDH examined. Human not HeLa
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:10,12 D13S317:11,12 D7S820:10,11 D16S539:11 VWA:19,22 TH01:9 AM:X TPOX:9,11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO	CO2 Conc.	5 %
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB1142	Cell Name	HEC-265
LOT No.	06132013	Lot Specification	distribution
Medium	Eagle's minimal essential medium with 15% fetal calf serum. (FBS; GIBCO Cat. No. #10099)	Temperature	37C
Cell Density at Seeding	0.6-1.3 x10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.1% trypisn and 0.02% EDTA.
Doubling Time		Cell Number in Vial (cells/1ml)	3.3 x 10^6 cells/1mL
Viability at cell freezing (%)		Antibiotics Used	free
Passage Number	P189	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,12 D13S317:11,12,13 D7S820:10,11 D16S539:11 VWA:19,21,22,23 TH01:9 AM:X TPOX:9,11 CSF1PO:11,12
Adhesion		Exoteric Gene
Medium for Freezing	Culture medium with 10% DMSO	CO2 Conc.	5%
Viability immediately after thawing (%)	94.4	Additional information

Cell No.	JCRB1142	Cell Name	HEC-265
LOT No.	12152017	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012).	Temperature	37 C
Cell Density at Seeding	1-2 x10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.1% trypisn.
Doubling Time	approx. 32 hrs.	Cell Number in Vial (cells/1ml)	2.8 x 10^6
Viability at cell freezing (%)	85	Antibiotics Used	free
Passage Number	P189	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	85	Additional information

Cell No.	JCRB1142	Cell Name	HEC-265
LOT No.	08062020	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	7 - 9 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 30 hrs.	Cell Number in Vial (cells/1ml)	2.7 x 10^6
Viability at cell freezing (%)	approx. 10	Antibiotics Used	free
Passage Number	P189	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	5% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	96	Additional information

Cell No.	JCRB1142	Cell Name	HEC-265
LOT No.	09192023	Lot Specification	distribution
Medium	Eagle's minimum essential medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 107322)	Temperature	37 C
Cell Density at Seeding	1.4 - 1.7 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time	approx. 25-28 hrs.	Cell Number in Vial (cells/1ml)	2.5 x 10^6
Viability at cell freezing (%)	88.7	Antibiotics Used	free
Passage Number	P190	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10,12 D13S317:11,12 D7S820:10,11 D16S539:11 VWA:19,22 TH01:9 AM:X TPOX:9,11 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM	CO2 Conc.	5%
Viability immediately after thawing (%)	91.6	Additional information