JCRB1145 HEC-50B Cell information
Cell type:general cells
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JCRB No.
JCRB1145
Cell Name
HEC-50B
Profile
Human endometrioid adenocarcinoma (G3) from japanese patient.
Other Name
HEC-50
Animal
human
Strain
Genus
Homo
Species
sapiens
Sex
F
Age
Identity
available
Tissue for Primary Cancer
uterus
Case history
Metastasis
No
Tissue Metastasized
Genetics
endometrial carcinoma
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
endometrial carcinoma
Classify
tumor
Established by
Hamano, M. & Kuramoto, H.
Registered by
Kuramoto, H.
Regulation for Distribution
Comment
Year
2005
Medium
Eagle's minimal essential medium with 15% fetal calf serum.
Methods for Passages
Harvested with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage
1-2 x 10^5 cells/mL
Race
Japanese
CO2 Conc.
5 %
Tissue Sampling
peritoneal fluid
Tissue Type
endometrioid adenoca. (uterine corpus)
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
tested
Detected RNA Virus
tested
CMV
-
parvoB19
-
HCV
-
HTLV-1
-
EBV
-
HBV
-
HIV-1
-
HTLV-2
-
HHV6
-
HTLV-1
-
HIV-2
-
HAV
-
HHV7
-
HTLV-2
-
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV
-
HIV-1
-
JCV
-
HIV-2
-
ADV
-
HPV18
-
Notes
Reference
Pubmed id:15053063 Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines. Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H Eur J Gynaecol Oncol. 2004;25(1):55-60
Pubmed id:2064198 Establishment and characterization of human endometrial cancer cell lines. Kuramoto H,Nishida M,Morisawa T,Hamano M,Hata H,Kato Y,Ohno E,Iida T Ann N Y Acad Sci. 1991;622():402-21
Pubmed id:269609 Cytogenetic studies of human endometrial carcinomas by means of tissue culture. Kuramoto H,Hamano M Acta Cytol. 1977 Jul-Aug;21(4):559-65
Movies
LOT Information
Cell No.
JCRB1145
Cell Name
HEC-50B
LOT No.
03032006
Lot Specification
distribution
Medium
Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum
Temperature
37 C
Cell Density at Seeding
3.1x10^3 cells/sq.cm
Methods for Passages
Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time
NT
Cell Number in Vial (cells/1ml)
1.2x10^6
Viability at cell freezing (%)
92.7
Antibiotics Used
Free
Passage Number
p859
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
G6PD(typeB), NP, LDH examined. Human not HeLa
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
D5S818:8 D13S317:9 D7S820:12 D16S539:12 VWA:14 TH01:9 AM:X TPOX:9 CSF1PO:10,12
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
Culture medium with 5% DMSO
CO2 Conc.
5 %
Viability immediately after thawing (%)
Additional information
Cell No.
JCRB1145
Cell Name
HEC-50B
LOT No.
10182013
Lot Specification
distribution
Medium
Eagle's minimal essential medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10099).
Temperature
37 C
Cell Density at Seeding
3.6 - 5.3 x 10^4 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time
Cell Number in Vial (cells/1ml)
4.7 x 10^6 cells/1mL
Viability at cell freezing (%)
Antibiotics Used
free
Passage Number
P862
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
D5S818:8 D13S317:9 D7S820:12 D16S539:12 VWA:14 TH01:9 AM:X TPOX:9 CSF1PO:10,12
Adhesion
Exoteric Gene
Medium for Freezing
Culture medium with 5% DMSO
CO2 Conc.
5%
Viability immediately after thawing (%)
91.0
Additional information
Cell No.
JCRB1145
Cell Name
HEC-50B
LOT No.
07232024
Lot Specification
distribution
Medium
Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K)
Temperature
37 C
Cell Density at Seeding
0.9 - 1.0 x 10^5 cells/mL
Methods for Passages
Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time
approx. 41 hrs.
Cell Number in Vial (cells/1ml)
1.6 x 10^6
Viability at cell freezing (%)
98.1
Antibiotics Used
free
Passage Number
P862
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
D5S818:8 D13S317:9 D7S820:12 D16S539:12 VWA:14 TH01:9 AM:X TPOX:9 CSF1PO:10,12
Adhesion
Yes
Exoteric Gene
Medium for Freezing
10% DMSO, 20% FBS - EMEM
CO2 Conc.
5%
Viability immediately after thawing (%)
90.6
Additional information