JCRB1146 RSMG-1

Cell information

Important Notice(s)

Use and cultivation of RSMG-1 and RSMG-2 cell lines

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1146 Cell Name   RSMG-1
Profile   Stem cell like cell line from female rat submandibular gland. Other Name   
Animal   rat Strain   Wistar
Genus   Rattus Species   norvegicus
Sex   F Age   10-week-old
Identity   not done Tissue for Primary Cancer   submandibular gland
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Growth of the RSMG-1 cells are inhibited by activin A, while accelarated by follistatin. The RSMG-1 cells shows a stem cell like characteristics.
Classify   normal Established by   Furue,M.
Registered by   Furue,M. Regulation for Distribution   Must cite the reference, {5541}.
Comment   Co-operative research with Dr. Furue,M. recommended because of the special characteristics of the RSMG cells. Year   2004
Medium   Serum free MCDB153 medium (Research Inst. for Functional Peptides, Yamagata, Japan) with Supplements (See important notice). Methods for Passages   Cells are harvested after treatment with 0.05% trypsin in 0.04% EDTA then inactivated by 0.1% soybean trypsin inhibitor.
Cell Number on Passage   split ratio = 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   selection from whole body culture
Tissue Type   submandibular gland epithelial cells

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5584
Reference
Pubmed id:11401526 Activin A induces expression of rat Sel-1l mRNA, a negative regulator of notch signaling, in rat salivary gland-derived epithelial cells.
Furue M,Zhang Y,Okamoto T,Hata RI,Asashima M
Biochem Biophys Res Commun. 2001 Apr 6;282(3):745-9
Pubmed id:10937830 Isoleucine prevents rat salivary gland epithelial cells from apoptosis in serum-free culture.
Furue M,Okamoto T,Koshika S,Asashima M
In Vitro Cell Dev Biol Anim. 2000 May;36(5):287-9
Pubmed id:10476908 Effects of hepatocyte growth factor (HGF) and activin A on the morphogenesis of rat submandibular gland-derived epithelial cells in serum-free collagen gel culture.
Furue M,Okamoto T,Hayashi H,Sato JD,Asashima M,Saito S
In Vitro Cell Dev Biol Anim. 1999 Mar;35(3):131-5
Pubmed id:9719409 Hepatocyte growth factor regulates activin betaA mRNA in submandibular gland.
Furue M,Saito S
In Vitro Cell Dev Biol Anim. 1998 Jul-Aug;34(7):520-3

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LOT Information

Cell No.   JCRB1146 Cell Name   RSMG-1
LOT No.   03162006 Lot Specification   distribution
Medium   MCDB153+5F+F Temperature   37 C
Cell Density at Seeding   1.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.04% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   95.5 Antibiotics Used   Free
Passage Number   P7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined.Rat confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing FAF-BSA and 10% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1146 Cell Name   RSMG-1
LOT No.   04222017 Lot Specification   distribution
Medium   MCDB153(Sigma:M7403,including Sodium selenite ) +4F(Insulin,apo-Transferrin ,2-Mercaptoethanol ,Ethanolamine) + FGF-1 with collagen coated dish (See attached file1 and file2) Temperature   37 C
Cell Density at Seeding   1-2x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05%trypsin(GIBCO) and 0.04% EDTA and scraping.(Split ratio=1/3-1/4) medium change every other day
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.00x10^6
Viability at cell freezing (%)   94.4 Antibiotics Used   
Passage Number   P12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   culture medium containing 1mg/ml FAF-BSA with 10% DMSO CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   88.8
Additional information   collagen coated dish,medium change every other day, See attached file 1 and file 2,before culture
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