JCRB0401 HUH-6 Clone 5

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0401 Cell Name   HUH-6 Clone 5
Profile   Human hepatoblastoma cell line from an infant. Other Name   JTC38
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   infant
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   hepatoblastoma Metastasis   No
Tissue Metastasized    Genetics   hepatoblastoma, HBsAg negative, 16ug/ml CEA
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Produces albumin, prealbumin, fibrinogen ect.
Classify   tumor Established by   Doi,I. & Sato,J.
Registered by   Sato,J. Regulation for Distribution   No cmmercial purpose.
Comment   The cell line grow in non serum medium. α-feto protein production positive. This cell line was found to be contaminated by mycoplasmas when this cell line was deposited at JCRB Cell Bank. JCRB eliminated the mycoplasmal contamination by the treatment with anti-mycoplasma reagents, and current lots for distribution are mycoplasma-free. Year   1985
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   Treat cells with 0.2% trypsin and 0.02% EDTA.
Cell Number on Passage   More than 5x10^4 cells/cm^2 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1296
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 907070
Reference
Pubmed id:2845213Release of pancreatic secretory trypsin inhibitor from human hepatoblastoma cells on stimulation with cytokines.
Murata A,Ogawa M,Uda K,Matsuura N,Watanabe Y,Baba T,Mori T
Life Sci. 1988;43(15):1233-40
Pubmed id:2415243Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines proliferating in chemically defined medium.
Nakabayashi H,Taketa K,Yamane T,Oda M,Sato J
Cancer Res. 1985 Dec;45(12 Pt 1):6379-83
Pubmed id:6286115Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.
Nakabayashi H,Taketa K,Miyano K,Yamane T,Sato J
Cancer Res. 1982 Sep;42(9):3858-63
Pubmed id:57894Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma.
Doi I
Gan. 1976 Feb;67(1):1-10

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LOT Information

Cell No.   JCRB0401 Cell Name   
LOT No.   12142012 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   38 - 55 hrs Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   67.2 Antibiotics Used   free
Passage Number   P52 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0401 Cell Name   HUH-6 Clone 5
LOT No.   04102009 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   2.1x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%(GIBCO) trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   p56 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,11
D13S317:8,12
D7S820:11,12
D16S539:10,11
VWA:14,17
TH01:7,8
AM:X,Y
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0401 Cell Name   HUH-6 Clone5
LOT No.   05262008 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot484375) Temperature   37 C
Cell Density at Seeding   5 - 10 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   35 hrs Cell Number in Vial (cells/1ml)   2.6 x 10^6
Viability at cell freezing (%)   77 Antibiotics Used   free
Passage Number   P55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   93% Additional information   
Cell No.   JCRB0401 Cell Name   
LOT No.   04112001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovie serum Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.2% trypsin and 0.02% EDAT in Ca, Mg-free PBS
Doubling Time    Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   P54 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0401 Cell Name   HUH-6 Clone 5
LOT No.   11212000 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB2405). Temperature   37 C
Cell Density at Seeding   1.7 x 10^4 cells/sq.cm. Methods for Passages   Cells harvested after treatment with 0.2 % trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5 x 10^5
Viability at cell freezing (%)   80.4 Antibiotics Used   No use but MC210 for this culture only.
Passage Number   P51 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0401 Cell Name   HUH-6 Clone 5
LOT No.   03032014 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10091). Temperature   37C
Cell Density at Seeding   4.0 - 5.5 x 10^4 cells/mL Methods for Passages   Treat cells with 0.2% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.2 x 10^6 (cells/1
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P53 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:8,11
D13S317:8,12
D7S820:11,12
D16S539:10,11
VWA:14,17
TH01:7,8
AM:X,Y
TPOX:8
CSF1PO:10,12
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   85.9 Additional information   
Cell No.   JCRB0401 Cell Name   HUH-6 Clone 5
LOT No.   03102014 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   4.6 - 5.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   P55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95.2 Additional information   
Cell No.   JCRB0401 Cell Name   HUH-6 Clone 5
LOT No.   07222016 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   0.6 - 1.1 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 37 hrs Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   P55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   80 Additional information   
Cell No.   JCRB0401 Cell Name   HUH-6 Clone 5
LOT No.   08132020 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.9 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   82.9 Antibiotics Used   free
Passage Number   P55 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   75.9 Additional information   
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