JCRB1155 Yub625

Cell information

Important Notice(s)

POWEREDBY10 medium

不死化していないヒト体性幹細胞の分譲について(倫理的な取り扱い)

On the distribution of human non-immortalized somatic stem cells to oversea users (ethical ragulation).

Cell type:primary mesenchymal stem cell (View Pricing Information)

JCRB No.   JCRB1155 Cell Name   Yub625
Profile   Cell line established from polydactylous human fingers (finger). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   mesenchymal stem cell
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umazawa, A.
Registered by   Umazawa, A. Regulation for Distribution   
Comment    Year   2005
Medium   Poweredby10 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA.
Cell Number on Passage   3x10^5 cell/10cm dish Race   
CO2 Conc.   5% Tissue Sampling   polydactylous human fingers (finger
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3263
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LOT Information

Cell No.   JCRB1155 Cell Name   Yub625
LOT No.   05102006 Lot Specification   distribution
Medium   POWEREDBY10 Temperature   37 C
Cell Density at Seeding   4.9x10^3 cells/sq.cm Methods for Passages   Cells were harvested treatment with 0.25% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   93.4 Antibiotics Used   
Passage Number   P6 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined.Human,not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:12
D7S820:10,11
D16S539:9,10
VWA:14,18
TH01:6,9.3
AM:X,Y
TPOX:8,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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