JCRB1164 HSC-F

Cell information

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サル由来細胞株の培養方法（特に解凍操作）に関して

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1164 Cell Name   HSC-F
Profile   T-cell line (fetal spleen-derived lymphocytes) of cynomolgus monkey. Other Name   
Animal   monkey, Cynomolgus Strain   Cynomolgus
Genus   Macaca Species   fascicularis
Sex   * Age   fetal
Identity   not done Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   Positive for CD2, CD3, CD4, CD8a, CD25, CD28, CD29, CD38, CD40L, CD44, CD49d, CD58, CD69, CD70, CD80, CD95 & MHC-IIDR.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Scattered clumps observed in cell culture and this cell line is liable to apotosis due to overgrowth. Herpesvirus saimiri is latently infected, while no infectious virus is produced from this cell line.
Classify   transformed Established by   Akari,H.
Registered by   Akari,H. Regulation for Distribution   
Comment   This cell line dose not require hIL-2, but in case of poor condition of cell growth an addition of the hIL-2(50U/ml)will improve the cell growth. Year   2006
Medium   RPMI-1640 with 10% fetal bovine serum. Methods for Passages   suspension culture (dilute 1/5) in 10ml of T25 flask vertical culture.
Cell Number on Passage   1x10^6 cells/ml in 10mL of T25 Race   
CO2 Conc.   5 % Tissue Sampling   spleen-derived lymphocytes
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5485
Reference
Pubmed id:10370378Effects of SIVmac infection on peripheral blood CD4+CD8+ T lymphocytes in cynomolgus macaques.
Akari H,Nam KH,Mori K,Otani I,Shibata H,Adachi A,Terao K,Yoshikawa Y
Clin Immunol. 1999 Jun;91(3):321-9
Pubmed id:8610465In vitro immortalization of Old World monkey T lymphocytes with Herpesvirus saimiri: its susceptibility to infection with simian immunodeficiency viruses.
Akari H,Mori K,Terao K,Otani I,Fukasawa M,Mukai R,Yoshikawa Y
Virology. 1996 Apr 15;218(2):382-8

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LOT Information

Cell No.   JCRB1164 Cell Name   HSC-F
LOT No.   08212006 Lot Specification   distribution
Medium   RPMI1640 with with 10% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   8.4x10^6 Methods for Passages   Before cell dencity reaches to 1x10^6/ml,dilute to 2x10^5/ml
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6x10^6
Viability at cell freezing (%)   96.7 Antibiotics Used   Free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined ,Monkey,Cynomologus
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1164 Cell Name   HSC-F
LOT No.   08072006 Lot Specification   distribution
Medium   RPMI1640 with with 10% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   3.7x10^5 cells/sq.cm Methods for Passages   Before cell dencity reaches to 1x10^6/ml,dilute to 2x10^5/ml
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.8x10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined.Monkey,Cynomologus
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1 CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
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