この細胞株はヘルペスウイルスサイミリで不死化されており、本来は培地にIL-2の添加無しで増殖します。しかし、非常にデリケートな細胞株で、使用するFBSのロットによる増殖の差が大きく、JCRB細胞バンクで培養する際にIL-2添加なしでは増殖が悪かったのでIL-2添加して培養しました。したがって、HSP-250、HSP-239、HSR-5を培養する際には、IL-2を添加して培養してください。IL-2の添加量は解凍時には多め（100-50 U/mL）に、順調に増殖してきたら徐々に減らすことは可能です。詳細は各細胞株のロット情報を参照してください。さらに、この細胞株は非常に小型なので遠心は、1200-1300 rpmで8-10分間ほどすることをお勧めします。
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| JCRB No. |
JCRB1165 |
Cell Name |
HSP-239 |
| Profile |
Peripheral blood lymphocyte cell line of chimpanzee. |
Other Name |
|
| Animal |
chimpanzee |
Strain |
|
| Genus |
Pan |
Species |
troglodytes |
| Sex |
M |
Age |
7 |
| Identity |
available |
Tissue for Primary Cancer |
hemo-lymphocytic |
| Case history |
|
Metastasis |
No |
| Tissue Metastasized |
|
Genetics |
Positive for CD2,CD3, and/or CD8a |
| Life Span |
infinite |
Crisis PDL |
|
| Morphology |
lymphocyte-like |
Character |
Scattered clumps observed in cell culture and this cell line is liable to apotosis due to overgrowth. Herpesvirus saimiri is latently infected, while no infectious virus is produced from this cell line. |
| Classify |
transformed |
Established by |
Akari,H. |
| Registered by |
Akari,H. |
Regulation for Distribution |
|
| Comment |
This cell line is liable to apoptosis due to over growth. This line normally grows without addition of IL-2, in case of poor condition, addition of hIL-2 will highly improve the cell growth. This cell line is cultured with IL-2 in JCRB cell bank. |
Year |
2006 |
| Medium |
RPMI1640 with 10% fetal bovine serum, IL-2 (Interleukin-2, human) ~100U/mL. |
Methods for Passages |
suspension culture (dilute 1/5) in 10ml of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition. |
| Cell Number on Passage |
10^6cells/mL vertical culture |
Race |
|
| CO2 Conc. |
5% |
Tissue Sampling |
peripheral blood lymphocytes |
| Tissue Type |
|
