JCRB0406 PLC/PRF/5

Cell information

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JCRB No.   JCRB0406 Cell Name   PLC/PRF/5
Profile   hepatoma, HBs antigen-positive Other Name   Alexander
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   24
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history   malignant liver cancer with HBsAg positive. Metastasis   No
Tissue Metastasized    Genetics   Loss of D & G group chromosomes, with marker chr.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   G6PD=Type A. Tumors developed in hamster(imn.sup).
Classify   tumor Established by   Alexander,J.J.
Registered by   Sato,J. Regulation for Distribution   Not regulated
Comment   Grow in serum free medium. HBs antigen produced. Year   
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum. Methods for Passages   Cells are rinsed once with PBS(-) and treat with 0.2% trypsin and 0.02 % EDTA.
Cell Number on Passage   4 x 10^4 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV + HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes HB virus genome fragment was detected by realtime PCR. However, viral particles were not found (Br. J. Cancer, 34: 509 515 (1976)) in this cell line. In addition, this cell line has not been reported to produce HBV. The infectious HBV particles will not be produced by this cell line.

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0485
Reference
Pubmed id:63998Establishment of a continuously growing cell line from primary carcinoma of the liver.
Alexander JJ,Bey EM,Geddes EW,Lecatsas G
S Afr Med J. 1976 Dec 18;50(54):2124-8
Pubmed id:187208Hepatitis B surface antigen produced by a human hepatoma cell line.
MacNab GM,Alexander JJ,Lecatsas G,Bey EM,Urbanowicz JM
Br J Cancer. 1976 Nov;34(5):509-15

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LOT Information

Cell No.   JCRB0406 Cell Name   
LOT No.   02262010 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2.9 - 3.4 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 42 hrs Cell Number in Vial (cells/1ml)   9.6 x 10^5
Viability at cell freezing (%)   94.4 Antibiotics Used   free
Passage Number   P70 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0406 Cell Name   PLC/PRF/5
LOT No.   02122010 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.3-2.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2x10^6
Viability at cell freezing (%)   91.8 Antibiotics Used   free
Passage Number   p71 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:11,12
D7S820:9,11
D16S539:13
VWA:15,16
TH01:7,8
AM:X
TPOX:8
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0406 Cell Name   
LOT No.   01272003 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS lot; BioWhittaker 0S069F) Temperature   37 C
Cell Density at Seeding   3-10 x 10^4 cells.ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   37 hrs Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   79 Antibiotics Used   free
Passage Number   P70 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS in DMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0406 Cell Name   PLC/PRF/5
LOT No.   122195 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum (Flow.0070893) Temperature   37 C
Cell Density at Seeding   3.8x10^4 cells/sq.cm Methods for Passages   Rinsed cells once with PBS(-) and treated with 0.1% trypsin and 0.005% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.97x10^6
Viability at cell freezing (%)   87.6 Antibiotics Used   free
Passage Number   P67 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB0406 Cell Name   
LOT No.   112097 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (FCS lot; BioWhittaker 6M0121) Temperature   37 C
Cell Density at Seeding   4-5 x 10^4 cells/ml Methods for Passages   0.2% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6.1 x 10^5
Viability at cell freezing (%)   86.0 Antibiotics Used   free
Passage Number   P70 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type A), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0406 Cell Name   PLC/PRF/5
LOT No.   12142015 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (low glucose) with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   5.3 - 7.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 49 hrs Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P71 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   95 Additional information   
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