JCRB1168 NCR-G3

Cell information

Important Notice(s)

Cultivation of JCRB1166 NCR-G1, JCRB1167 NCR-G2, JCRB1168 NCR-G3, JCRB1175 NCR-G4

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1168 Cell Name   NCR-G3
Profile   Complex type germ cell tumor of human embryonic testis origin. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   
Identity   available Tissue for Primary Cancer   testis
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   complex type Character   
Classify   tumor Established by   Hata,J.
Registered by   Umezawa,A. Regulation for Distribution   
Comment    Year   2005
Medium   G031101 Methods for Passages   The cell is peeled off by pipetting. (It handles it as much as the human embryonic stem cell. )
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   human embryonic testis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3061
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LOT Information

Cell No.   JCRB1168 Cell Name   NCR-G3
LOT No.   09122006 Lot Specification   distribution
Medium   G031101(within serum and antibiotics:MED-SHIROTORI:Cat#31101) Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   The cell is peeled off by pipetting,after PBS(-) washing and treatment with Cell Dissociation Buffer enzyme-free,Hanks-based(Invitrogen Cat.No.13150-016)
Doubling Time   NT Cell Number in Vial (cells/1ml)   NT
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number   P19 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined.Human,not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:11
D7S820:11,13
D16S539:13
VWA:16,19
TH01:6
AM:X,Y
TPOX:8
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1168 Cell Name   NCR-G3
LOT No.   05122014 Lot Specification   distribution
Medium   G031101 medium (GlycoTechnica, Ltd.) Temperature   37 C
Cell Density at Seeding   Could not be counted (due to cell aggregates) Methods for Passages   The cell is peeled off by pipetting after PBS(-) washing and treatment with Cell Dissociation Buffer enzyme-free,Hanks-based (Invitrogen Cat.No.13150-016)
Doubling Time    Cell Number in Vial (cells/1ml)   Could not be counted.
Viability at cell freezing (%)   N.T. Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   N.T.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:11
D7S820:11,13
D16S539:13
VWA:16,19
TH01:6
AM:X,Y
TPOX:8
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   7.5% DMSO, 20% FBS - G031101 CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   N.T.
Additional information   
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