JCRB1170 SKNO-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1170 Cell Name   SKNO-1
Profile   a GM-CSF dependent cell line with an t8,21 chromosome translocation estabulished from a patient attacked by acute myeloid leukemia(AML) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   22
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   acute myeloblastic leukaemia (AML) Metastasis   No
Tissue Metastasized    Genetics   t(8;21), monosomy17, p53 mutation.
Life Span   infinite Crisis PDL   
Morphology   myeloblast Character   MPO+, CAE+, NBE-.
Classify   tumor Established by   
Registered by   Kawaguchi, R. Regulation for Distribution   
Comment    Year   2006
Medium   RPMI 1640 with 10% Fetal Bovine Serum and 10 ng/ml rhGM-CFS(recombinant human granurocyte-macrophage colonystimulating factor) Methods for Passages   
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   myeloblast

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2196
Reference
Pubmed id:9029185Glucocorticoids induce apoptosis in acute myeloid leukemia cell lines with A t(8;21) chromosome translocation.
Miyoshi H,Ohki M,Nakagawa T,Honma Y
Leuk Res. 1997 Jan;21(1):45-50
Pubmed id:7772516Establishment of a myeloid leukaemic cell line (SKNO-1) from a patient with t(8;21) who acquired monosomy 17 during disease progression.
Matozaki S,Nakagawa T,Kawaguchi R,Aozaki R,Tsutsumi M,Murayama T,Koizumi T,Nishimura R,Isobe T,Chihara K
Br J Haematol. 1995 Apr;89(4):805-11
Pubmed id:7972130Growth inhibition and induction of differentiation of t(8;21) acute myeloid leukemia cells by the DNA-binding domain of PEBP2 and the AML1/MTG8(ETO)-specific antisense oligonucleotide.
Sakakura C,Yamaguchi-Iwai Y,Satake M,Bae SC,Takahashi A,Ogawa E,Hagiwara A,Takahashi T,Murakami A,Makino K
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11723-7
Pubmed id:8136267Establishment of a leukaemic cell line from a patient with acquisition of chromosomal abnormalities during disease progression in myelodysplastic syndrome.
Nakagawa T,Matozaki S,Murayama T,Nishimura R,Tsutsumi M,Kawaguchi R,Yokoyama Y,Hikiji K,Isobe T,Chihara K
Br J Haematol. 1993 Nov;85(3):469-76
Pubmed id:2096594A case of epidural granulocytic sarcoma preceding acute leukemia.
Watanabe M,Sashikata T,Kizaki T,Fujiwara T,Ugai K,Nakagawa T
Acta Pathol Jpn. 1990 Dec;40(12):922-6

Images



LOT Information

Cell No.   JCRB1170 Cell Name   
LOT No.   06112012 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS, GIBCO Cat. # 10099) and 10 ng/mL recombinant human GM-CSF Temperature   37 C
Cell Density at Seeding   2.9 - 4.3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 38 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   90.8 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1170 Cell Name   SKNO-1
LOT No.   08192006 Lot Specification   distribution
Medium   RPMI1640 with 10% heat inactivated fetal bovine serum and 10ng/ml rhGM-CFS(recombinant human granurocyte-macrophage colonystimulating facter) Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   subculture by dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   NT
Viability at cell freezing (%)   NT Antibiotics Used   
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined.Human,not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
D13S317:8,11
D7S820:10,12
D16S539:10,11
VWA:14,17
TH01:7
AM:X
TPOX:8,9
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   NT CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1170 Cell Name   SKNO-1
LOT No.   02032016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) and 10 ng/ml recombinant human GM-CFS Temperature   37 C
Cell Density at Seeding   4.8 - 7.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   52 - 57 hrs Cell Number in Vial (cells/1ml)   5.6 x 10^6
Viability at cell freezing (%)   87.1 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   87.1 Additional information   
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