JCRB1177 HSP-250

Cell information

Important Notice(s)

サル由来細胞株の培養方法（特に解凍操作）に関して

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1177 Cell Name   HSP-250
Profile   Peripheral blood lymphocyte cell line of chimpanzee. Other Name   
Animal   chimpanzee Strain   
Genus   Pan Species   troglodytes
Sex   F Age   6
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   No
Tissue Metastasized    Genetics   Positive for CD2,CD3, nd/or CD8a.
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Scattered clumps observed in cell culture and this cell line is liable to apotosis due to overgrowth. Herpesvirus saimiri is latently infected, while no infectious virus is produced from this cell line.
Classify   transformed Established by   Akari,H.
Registered by   Akari,H. Regulation for Distribution   
Comment   This cell line is liable to apoptosis due to over growth. This line　normally grows without addition of IL-2, in case of poor condition, addition of hIL-2 will highly improve the cell growth. This cell line is cultured with IL-2 in JCRB cell bank. Year   2006
Medium   RPMI1640 with 10% fetal bovine serum, IL-2 (interleukin-2, human) ~100 U/mL. Methods for Passages   suspension culture (dilute 1/5) in 10mL of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition.
Cell Number on Passage   10^6cells/mL vertical culture Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood lymphocytes
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2519
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LOT Information

Cell No.   JCRB1177 Cell Name   HSP-250
LOT No.   02282007 Lot Specification   distribution2
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) and IL-2(25u/ml)(Roche Interleukin-2,human) Temperature   37 C
Cell Density at Seeding   2.5 - 5.4 x 10^5 cells/mL Methods for Passages   Before cell dencity reaches to 1x10^6/ml,dilute to 3x10^5/ml
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.0x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   p13* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1177 Cell Name   HSP-250
LOT No.   12012006 Lot Specification   distribution
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) and IL-2(25u/ml)(Roche Interleukin-2,human) Temperature   37 C
Cell Density at Seeding   4.4x^5 cells/sq.cm Methods for Passages   Before cell dencity reaches 1x10^6/ml,dilute to 3x10^5/ml
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.3x10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   p11* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   48 Chromosome Information   48/49 49/1 (50)
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
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