JCRB1178 KUM5

Cell information

Important Notice(s)

M061101 medium

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1178 Cell Name   KUM5
Profile   Osteoblastic cell line of mouse C3H. Other Name   
Animal   mouse Strain   C3H/He
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   bone marrow
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2006
Medium   M061101 Methods for Passages   0.25% Trypsin - 0.53 mM EDTA
Cell Number on Passage   2 x 10^5 cells / 10 cm dish Race   
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   Osteoblasts

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5151
Reference
Pubmed id:15996824 Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts.
Matsumoto S,Shibuya I,Kusakari S,Segawa K,Uyama T,Shimada A,Umezawa A
Biochim Biophys Acta. 2005 Aug 30;1725(1):57-63
Pubmed id:1373147 Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.
Umezawa A,Maruyama T,Segawa K,Shadduck RK,Waheed A,Hata J
J Cell Physiol. 1992 Apr;151(1):197-205

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LOT Information

Cell No.   JCRB1178 Cell Name   KUM5
LOT No.   11142006 Lot Specification   distribution
Medium   M061101(MED-SHIROTORI:Cat#61101) Temperature   37 C
Cell Density at Seeding   2.2x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   
Passage Number   p14 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,NP,LDH were examined.Mouse confirmed.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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