JCRB1182 NCC-RbC-51

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1182 Cell Name   NCC-RbC-51
Profile   Human retinoblastoma cell line. The growth is very slow. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   4.8
Identity   available Tissue for Primary Cancer   neural
Case history   Familial bilateral retinoblastoma, cervical lymph node metastasis. Metastasis   Yes
Tissue Metastasized    Genetics   familial bilateral retinoblastoma
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   
Classify   tumor Established by   Inomata, M.
Registered by   Inomata, M. Regulation for Distribution   
Comment    Year   2006
Medium   CMRL1066 medium with 10-15% fetal bovine serum (Glutamine (final 100 μM) and Sodium pyruvate (final 200 μM). Methods for Passages   Simple dilution. split ratio=1/5. Subculture every 1 week.
Cell Number on Passage   1 x 10^6 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   cervical lymph node metastasis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3048
Reference
Pubmed id:8263010Culture of retinoblastoma cells from clinical specimens: growth-promoting effect of 2-mercaptoethanol.
Inomata M,Kaneko A,Saijo N,Tokura S
J Cancer Res Clin Oncol. 1994;120(3):149-55
Pubmed id:3115934Chemosensitivity profiles of primary and cultured human retinoblastoma cells in a human tumor clonogenic assay.
Inomata M,Kaneko A
Jpn J Cancer Res. 1987 Aug;78(8):858-68

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LOT Information

Cell No.   JCRB1182 Cell Name   NCC-RbC-51
LOT No.   02202007 Lot Specification   distribution
Medium   RPMI1640(GIBCO) with 20% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   2.9x10^5 cells/sq.cm Methods for Passages   Suspension culture.Split ratio=1/2.Subculture every 1week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.9x10^6
Viability at cell freezing (%)   91.4 Antibiotics Used   free
Passage Number   p13* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:11,12
D7S820:12
D16S539:10,12
VWA:14
TH01:9
AM:X,Y
TPOX:9,11
CSF1PO:13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker:BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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