JCRB1193 HEC-1-B

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1193 Cell Name   HEC-1-B
Profile   Endometrioid adenocarcinoma (G2) from human uterus body. Other Name   HEC-BE
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   No
Tissue Metastasized    Genetics   endometrial carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endometrial carcinoma
Classify   tumor Established by   Kuramoto,H.
Registered by   Kuramoto,H. Regulation for Distribution   
Comment   HEC-1-B is the same cell line with the HEC-BE. Year   2005
Medium   Eagle's minimal essencial medium with 10% fetal calf serum. Methods for Passages   Harvested with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1-2 x 10^5 cells/mL Race   Japanese
CO2 Conc.   5 % Tissue Sampling   uterine corpus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0294
Reference
Pubmed id:15053063 Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60
Pubmed id:269609 Cytogenetic studies of human endometrial carcinomas by means of tissue culture.
Kuramoto H,Hamano M
Acta Cytol. 1977 Jul-Aug;21(4):559-65
Pubmed id:862644 Establishment and characterization of the cell-line of a human edometrial adenoacanthoma.
Kuramoto H,Hamano M
Eur J Cancer. 1977 Mar 29;13(3):253-9

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LOT Information

Cell No.   JCRB1193 Cell Name   HEC-1-B
LOT No.   07172007 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(Intergen RB52305) Temperature   37 C
Cell Density at Seeding   2.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   p122 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:11
D7S820:9,11
D16S539:11,12
VWA:18
TH01:6,7
AM:X
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1193 Cell Name   HEC-1-B
LOT No.   01202016 Lot Specification   distribution
Medium   Eagle's minimum essencial medium with 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   3.2 - 4.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 46 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   86.7 Antibiotics Used   free
Passage Number   P125 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.7
Additional information   
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