JCRB0418 dRLN-4
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0418 | Cell Name | dRLN-4 |
|---|---|---|---|
| Profile | liver cell line, 3'-methyl-4-dimethylaminoazobenzene induced, (Donryu rat) | Other Name | |
| Animal | rat | Strain | Donryu |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | liver | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | liver after treatment by 0.06 % DAB | |
| Life Span | Crisis PDL | ||
| Morphology | Character | liver after treatment by 0.06 % DAB | |
| Classify | Established by | Hoshika,T. & Sato,J. | |
| Registered by | Sato,J. | Regulation for Distribution | |
| Comment | Year | 1986 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum. | Methods for Passages | Cells treated with 0.2 % trypsin and 0.02% EDTA at subculture. |
| Cell Number on Passage | More than 1x10^4 cells/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | liver |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Images |
|---|
       |
LOT Information
| Cell No. | JCRB0418 | Cell Name | |
|---|---|---|---|
| LOT No. | 020299 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium + 10% fetal calf serum (Biowhittaker 6M0121) | Temperature | 37 C |
| Cell Density at Seeding | 8.0 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | ca 30hrs | Cell Number in Vial (cells/1ml) | 4.4 x 10^6 |
| Viability at cell freezing (%) | 85% | Antibiotics Used | free |
| Passage Number | P13 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as rat by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |