JCRB0426 HuH28

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0426 Cell Name   HuH28
Profile   Human liver, bile duct carcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   37-year-old
Identity   available Tissue for Primary Cancer   liver, gallbladder
Case history    Metastasis   
Tissue Metastasized    Genetics   bile duct carcinoma (liver)
Life Span   infinite Crisis PDL   
Morphology    Character   bile duct carcinoma (liver)
Classify   tumor Established by   Kusaka,Y. et al.
Registered by   Sato,J., Tokiwa,T. Regulation for Distribution   
Comment   Doubling time is very slow (ca. 1 week). Year   1989
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells treated with 0.2% trypsin and 0.02% EDTA for 5 min at 37 C.
Cell Number on Passage   5-15 x 10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   liver bile duct
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2955
Reference
Pubmed id:2852388Establishment and characterization of a cell line from a human cholangiocellular carcinoma.
Kusaka Y,Tokiwa T,Sato J
Res Exp Med (Berl). 1988;188(5):367-75

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LOT Information

Cell No.   JCRB0426 Cell Name   HuH28
LOT No.   01062011 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 2.5 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Growth is very slow. If islands of cells are formed, the growth in the islands retard. In such case, please replate the cells.
Doubling Time   approx. 88 hrs. Cell Number in Vial (cells/1ml)   6.1 x 10^5
Viability at cell freezing (%)   89.7 Antibiotics Used   free
Passage Number   Unknown (18 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   93% Additional information   
Cell No.   JCRB0426 Cell Name   
LOT No.   09082006 Lot Specification   distribution
Medium   RPMI1640 mdeium with 10% fetal bovine serum (FBS lot; GIBCO4218333S) Temperature   37 C
Cell Density at Seeding   1 - 2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1 week Cell Number in Vial (cells/1ml)   5.6 x 10^5
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   Unknown (18 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as humna by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0426 Cell Name   HuH28
LOT No.   06302014 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.1 - 2.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   7.0 x 10^5
Viability at cell freezing (%)   82 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmes as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,12
D13S317:9,12
D7S820:10,11
D16S539:9
VWA:17
TH01:9
AM:X
TPOX:8
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   82 Additional information   
Cell No.   JCRB0426 Cell Name   HuH28
LOT No.   07222016 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   very slow (55 - 128 hrs). Cell Number in Vial (cells/1ml)   4.4 x 10^5
Viability at cell freezing (%)   88.0 Antibiotics Used   free
Passage Number   Unknown (18 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Cell No.   JCRB0426 Cell Name   HuH28
LOT No.   10022018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.1 - 4.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   very slow (5 days) Cell Number in Vial (cells/1ml)   2.1 - 4.1 x 10^4 cells/mL
Viability at cell freezing (%)   91.2 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85.3 Additional information   
Cell No.   JCRB0426 Cell Name   HuH28
LOT No.   01052024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.5 - 3.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   slow (> 91 hrs) Cell Number in Vial (cells/1ml)   8.4 x 10^5
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (19 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,12
D13S317:9,12
D7S820:10,11
D16S539:9
VWA:17
TH01:9
AM:X
TPOX:8
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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