JCRB0501 TIG-1-20

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0501 Cell Name   TIG-1-20
Profile   fetal lung, normal diploid fibroblast, TIG-1, PDL=about 20 Other Name   TIG-1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   fetal lung, (F)
Life Span    Crisis PDL   
Morphology    Character   fetal lung, (F)
Classify    Established by   Tky.Inst.Geront.
Registered by   Matsuo,M. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Subculture every 7 days. Split ratio, 1/4. 0.25% trypsin, 10-15 min.
Cell Number on Passage   6-8x10^4 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   fetus lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3181
Reference
Pubmed id:1647360 Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter.
Takekoshi M,Maeda-Takekoshi F,Ihara S,Sakuma S,Watanabe Y
Gene. 1991 May 30;101(2):209-13
Pubmed id:3561390 The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck.
Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K
Mol Cell Biol. 1987 Jan;7(1):237-43
Pubmed id:7389832 A new human diploid cell strain, TIG-1, for the research on cellular aging.
Ohashi M,Aizawa S,Ooka H,Ohsawa T,Kaji K,Kondo H,Kobayashi T,Noumura T,Matsuo M,Mitsui Y,Murota S,Yamamoto K,Ito H,Shimada H,Utakoji T
Exp Gerontol. 1980;15(2):121-33

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LOT Information

Cell No.   JCRB0501 Cell Name   
LOT No.   08172010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   5.2 - 6.8 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   01132009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. No. 10099, Lot 484375). This lot of cells were frozen using DMSO. Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 21 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   01302008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). This stock was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio = 1:4
Doubling Time   32 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   20.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   01202006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO4218333S). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   approx. 1:4 split. 6-8 x 10^4 cells/ml. Methods for Passages   0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 day Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (P6 at bank) PDL   21
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   R012 Lot Specification   distribution
Medium   MEM + 10% FCS (this ampule was freezed using glycerol Temperature   37
Cell Density at Seeding   6-8x10^4 Methods for Passages   Subculture every 7 days. Split ratio 1:4. 0.25% trypsin, 10-15 min.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   24PDL
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   R013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% FCS This stock was freezed using glycerol. Temperature   37 C
Cell Density at Seeding    Methods for Passages   Treat cells with 0.25% trypsin for 10-15 min. Subculture every 7 days. Split ratio, 1:4.
Doubling Time    Cell Number in Vial (cells/1ml)   7.4 x 10^6
Viability at cell freezing (%)   70.0 Antibiotics Used   100 ug/ml streptmycin and 100 unit/ml penicillin
Passage Number    PDL   20
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   R014 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% FBS This stock was frozen using glycerol. Temperature   37 C
Cell Density at Seeding   6-8 x 10^4 cells/cm2 Methods for Passages   Subculture every 7 days. Split ratio, 1:4 Treat with 0.25% trypsin for 10-15 min
Doubling Time    Cell Number in Vial (cells/1ml)   7.0 x 10^6
Viability at cell freezing (%)   85.7 Antibiotics Used   
Passage Number    PDL   23
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   R015 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. This lot of ampoule was frozen using glycerol. Temperature   37 C
Cell Density at Seeding   split ratio 1:4 Methods for Passages   0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   6.3 x 10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   100 ug/ml streptomycin and 100 units/ml penicillin
Passage Number    PDL   23
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture medium containing 10% glycerol. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   04272015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   4.0 - 8.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   9.6 x 10^5
Viability at cell freezing (%)   90.6 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90.6
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   06142016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS lot; Sigma 12J396). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   5.7 - 6.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   80.2 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80.2
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   05302017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   4.8 - 7.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   64.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   64.0
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   07092018 Lot Specification   distribution
Medium   Eagle's minimam essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   approx. 6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   9.8 x 10^5
Viability at cell freezing (%)   86.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   20.9
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.7
Additional information   
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