JCRB0501 TIG-1-20

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0501 Cell Name   TIG-1-20
Profile   fetal lung, normal diploid fibroblast, TIG-1, PDL=about 20 Other Name   TIG-1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   fetal lung, (F)
Life Span    Crisis PDL   PDL=67 (av
Morphology    Character   fetal lung, (F)
Classify    Established by   Tky.Inst.Geront.
Registered by   Matsuo,M. Regulation for Distribution   
Comment    Year   1985
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Subculture every 7 days. Split ratio, 1/4. 0.25% trypsin, 10-15 min.
Cell Number on Passage   6-8x10^4 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   fetus lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3181
Reference
Pubmed id:1647360 Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter.
Takekoshi M,Maeda-Takekoshi F,Ihara S,Sakuma S,Watanabe Y
Gene. 1991 May 30;101(2):209-13
Pubmed id:3561390 The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck.
Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K
Mol Cell Biol. 1987 Jan;7(1):237-43
Pubmed id:7389832 A new human diploid cell strain, TIG-1, for the research on cellular aging.
Ohashi M,Aizawa S,Ooka H,Ohsawa T,Kaji K,Kondo H,Kobayashi T,Noumura T,Matsuo M,Mitsui Y,Murota S,Yamamoto K,Ito H,Shimada H,Utakoji T
Exp Gerontol. 1980;15(2):121-33

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LOT Information

Cell No.   JCRB0501 Cell Name   
LOT No.   08172010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   5.2 - 6.8 x 10^4 cells/mL Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 1.5 days Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   01132009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. No. 10099, Lot 484375). This lot of cells were frozen using DMSO. Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 21 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   01302008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). This stock was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio = 1:4
Doubling Time   32 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   20.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   01202006 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO4218333S). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   approx. 1:4 split. 6-8 x 10^4 cells/ml. Methods for Passages   0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 day Cell Number in Vial (cells/1ml)   1.3 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (P6 at bank) PDL   21
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   R012 Lot Specification   distribution
Medium   MEM + 10% FCS (this ampule was freezed using glycerol Temperature   37
Cell Density at Seeding   6-8x10^4 Methods for Passages   Subculture every 7 days. Split ratio 1:4. 0.25% trypsin, 10-15 min.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   24PDL
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   R013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% FCS This stock was freezed using glycerol. Temperature   37 C
Cell Density at Seeding    Methods for Passages   Treat cells with 0.25% trypsin for 10-15 min. Subculture every 7 days. Split ratio, 1:4.
Doubling Time    Cell Number in Vial (cells/1ml)   7.4 x 10^6
Viability at cell freezing (%)   70.0 Antibiotics Used   100 ug/ml streptmycin and 100 unit/ml penicillin
Passage Number    PDL   20
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   R014 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% FBS This stock was frozen using glycerol. Temperature   37 C
Cell Density at Seeding   6-8 x 10^4 cells/cm2 Methods for Passages   Subculture every 7 days. Split ratio, 1:4 Treat with 0.25% trypsin for 10-15 min
Doubling Time    Cell Number in Vial (cells/1ml)   7.0 x 10^6
Viability at cell freezing (%)   85.7 Antibiotics Used   
Passage Number    PDL   23
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   
LOT No.   R015 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. This lot of ampoule was frozen using glycerol. Temperature   37 C
Cell Density at Seeding   split ratio 1:4 Methods for Passages   0.25% trypsin
Doubling Time    Cell Number in Vial (cells/1ml)   6.3 x 10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   100 ug/ml streptomycin and 100 units/ml penicillin
Passage Number    PDL   23
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   Culture medium containing 10% glycerol. CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   04272015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   4.0 - 8.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   9.6 x 10^5
Viability at cell freezing (%)   90.6 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90.6
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   06142016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS lot; Sigma 12J396). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   5.7 - 6.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   80.2 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80.2
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   05302017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   4.8 - 7.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   3.4 x 10^6
Viability at cell freezing (%)   64.0 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   23.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   64.0
Additional information   
Cell No.   JCRB0501 Cell Name   TIG-1-20
LOT No.   07092018 Lot Specification   distribution
Medium   Eagle's minimam essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   approx. 6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   9.8 x 10^5
Viability at cell freezing (%)   86.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   20.9
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.7
Additional information   
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