JCRB0504 TIG-1-50
Cell information
Important Notice(s)Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0504 | Cell Name | TIG-1-50 |
|---|---|---|---|
| Profile | fetal lung, normal diploid fibroblast, TIG-1, PDL=about 50 | Other Name | TIG-1 |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | lung | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | fetal lung, (F) | |
| Life Span | Crisis PDL | 67 (averag | |
| Morphology | fibroblast-like | Character | fetal lung, (F) |
| Classify | Established by | Tky.Inst.Geront. | |
| Registered by | Matsuo,M. | Regulation for Distribution | |
| Comment | Year | 1985 | |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | Subculture every 7 days. Split ratio, 1/4. 0.25% trypsin, 10-15 min. |
| Cell Number on Passage | 2-4x10^4 cells/cm^2 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | fetus lung |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1647360 | Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter. Takekoshi M,Maeda-Takekoshi F,Ihara S,Sakuma S,Watanabe Y Gene. 1991 May 30;101(2):209-13 |
| Pubmed id:3561390 | The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K Mol Cell Biol. 1987 Jan;7(1):237-43 |
| Pubmed id:7389832 | A new human diploid cell strain, TIG-1, for the research on cellular aging. Ohashi M,Aizawa S,Ooka H,Ohsawa T,Kaji K,Kondo H,Kobayashi T,Noumura T,Matsuo M,Mitsui Y,Murota S,Yamamoto K,Ito H,Shimada H,Utakoji T Exp Gerontol. 1980;15(2):121-33 |
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LOT Information
| Cell No. | JCRB0504 | Cell Name | |
|---|---|---|---|
| LOT No. | 03082006 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (FBS Lot; GIBCO 4218333S). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 6 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA |
| Doubling Time | approx. 1.5 days | Cell Number in Vial (cells/1ml) | 1.2 x 10^6 |
| Viability at cell freezing (%) | 92.2 | Antibiotics Used | free |
| Passage Number | Unknown (P18 at bank) | PDL | 50.0 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD, LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0504 | Cell Name | TIG-1-50 |
|---|---|---|---|
| LOT No. | 08222018 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | approx. 6 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 26 hrs | Cell Number in Vial (cells/1ml) | 1.1 x 10\6 |
| Viability at cell freezing (%) | 81.5 | Antibiotics Used | free |
| Passage Number | Unknown (18 at bank) | PDL | 48.9 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:9,10 D13S317:11,12 D7S820:8,11 D16S539:11,12 VWA:14,17 TH01:7 AM:X TPOX:11 CSF1PO:12,13 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 81.5 | Additional information |