JCRB0518 WI-38-40

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0518 Cell Name   WI-38-40
Profile   Normal primary cells from lung as well as JCRB0517. Now only the JCRB0518 with PDL 40 is available. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   lung
Case history   no Metastasis   
Tissue Metastasized    Genetics   lung, normal diploid fibroblast
Life Span    Crisis PDL   
Morphology    Character   lung, normal diploid fibroblast
Classify   new Established by   Hayflick,L.
Registered by   Ooka,H. Regulation for Distribution   
Comment    Year   1989
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   Wash in CMF-PBS.Add 0.25% trypsin.Keep at 37C for 10-15 min. Suspend cells in medium.
Cell Number on Passage   split ratio=1/4 Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2813
Reference
Pubmed id:4290661 SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.
Girardi AJ,Weinstein D,Moorhead PS
Ann Med Exp Biol Fenn. 1966;44(2):242-54
Pubmed id:14315085 THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.
HAYFLICK L
Exp Cell Res. 1965 Mar;37():614-36
Pubmed id:14288316 SV40-INDUCED TRANFORMATION OF HUMAN DIPLOID CELLS: CRISIS AND RECOVERY.
GIRARDI AJ,JENSEN FC,KOPROWSKI H
J Cell Comp Physiol. 1965 Feb;65():69-83
Pubmed id:13905660 Preparation of poliovirus vaccines in a human fetal diploid cell strain.
HAYFLICK L,PLOTKIN SA,NORTON TW,KOPROWSKI H
Am J Hyg. 1962 Mar;75():240-58

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LOT Information

Cell No.   JCRB0518 Cell Name   
LOT No.   04102007 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). This lot of cells was frozen in DMSO-containing medium. Temperature   37 C
Cell Density at Seeding   2-3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Split ratio=1:4 is recommended.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   3.4 x 10^5
Viability at cell freezing (%)   82.1 Antibiotics Used   free
Passage Number   Unknown PDL   40.8 PD
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by G6PD (typeB), NP, LD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0518 Cell Name   
LOT No.   R381 Lot Specification   distribution
Medium   MEM +10% FBS (this ampule was freezed using DMSO) Temperature   37
Cell Density at Seeding    Methods for Passages   Wash in CMF-PBS.Add 0.25% trypsin.Keep at 37C for 10-15 min. Suspend cells in medium.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   penicillin(100 units/ml)/streptomycin(100ug/ml)
Passage Number    PDL   41
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0518 Cell Name   
LOT No.   R382 Lot Specification   distribution
Medium   Eagle's MEM with 10% FBS (JCRB No.0517=0518)This stock was freezed using glycerol. Temperature   37 C
Cell Density at Seeding   split 1:4 Methods for Passages   Wash in CMF-PBS. Add 0.25% trypsin. Keep at 37 C for 10-15 min. Suspend cells in medium.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   100 ug/ml streptmycin and 100 units/ml penicilline
Passage Number    PDL   PDL40
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0518 Cell Name   WI-38-40
LOT No.   11022015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2.0 - 5.3 x 10^4 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   36 - 42 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   80.5 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   40
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80.5
Additional information   
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