JCRB0527 TIG-102
Cell information
Important Notice(s)Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0527 | Cell Name | TIG-102 |
|---|---|---|---|
| Profile | Normal human skin fibroblast cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 97 |
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | normal | Metastasis | |
| Tissue Metastasized | Genetics | skin, normal diploid fibroblast | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | skin, normal diploid fibroblast |
| Classify | normal | Established by | Yamamoto,K. |
| Registered by | Ooka,H | Regulation for Distribution | |
| Comment | Life span = PD41 | Year | 1989 |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum | Methods for Passages | Wash in CMF-PBS.Add 0.25% trypsin.Incubate at 37C for 10-15min.Suspend cells in medium. |
| Cell Number on Passage | 6-7x10^4 cells/cm^2 | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | skin |
| Tissue Type | normal diploid (2n=46, XX) |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
| Cell No. | JCRB0527 | Cell Name | |
|---|---|---|---|
| LOT No. | 05232007 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 484375). This lot was frozen using DMSO. | Temperature | 37 C |
| Cell Density at Seeding | 2-3 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA. Split ratio = 1:4 |
| Doubling Time | approx. 70 hrs | Cell Number in Vial (cells/1ml) | 4.3 x 10^4 |
| Viability at cell freezing (%) | 68.6 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | 28.7 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD, LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0527 | Cell Name | TIG-102 |
|---|---|---|---|
| LOT No. | 07072014 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | 1.3 - 4.2 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | Cell Number in Vial (cells/1ml) | 4.8 x 10^5 | |
| Viability at cell freezing (%) | 88 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | 28.7 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 88 | Additional information |
| Cell No. | JCRB0527 | Cell Name | TIG-102 |
|---|---|---|---|
| LOT No. | 05242022 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) | Temperature | 37 C |
| Cell Density at Seeding | 1.7 - 4.5 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 3 days | Cell Number in Vial (cells/1ml) | 3.8 x 10^5 |
| Viability at cell freezing (%) | 98.3 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | 28.6 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12,13 D13S317:9,11 D7S820:8,9 D16S539:9,12 VWA:17,18 TH01:7,9 AM:X TPOX:8,11 CSF1PO:11,12 |
|
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | approx. 100 | Additional information |