JCRB0532 TIG-107

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0532 Cell Name   TIG-107
Profile   skin, normal diploid fibroblast Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   81
Identity   available Tissue for Primary Cancer   skin
Case history    Metastasis   
Tissue Metastasized    Genetics   skin, normal diploid fibroblast
Life Span    Crisis PDL   
Morphology    Character   skin, normal diploid fibroblast
Classify   normal Established by   Tky.Inst.Geront.
Registered by   Ooka,H. Regulation for Distribution   
Comment    Year   1989
Medium   Eagle's minimal essential medium with 10% fetal bovine serum Methods for Passages   Wash in CMF-PBS.Add 0.25% trypsin.Incubate at 37C for 10-15min.Suspend cells in medium.
Cell Number on Passage   5x10^4 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3192
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LOT Information

Cell No.   JCRB0532 Cell Name   TIG-107
LOT No.   01182008 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine seru (FBS lot; GIBCO 484375). This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   2 - 4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   45 hrs Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   18.1
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   88
Additional information   
Cell No.   JCRB0532 Cell Name   TIG-107
LOT No.   11202015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   1.0 - 2.9 x 10^5 cell/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   29 - 63 hrs Cell Number in Vial (cells/1ml)   7.5 x 10^5
Viability at cell freezing (%)   89.6 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   26.1
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS -EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.6
Additional information   
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