JCRB0603 V79

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0603 Cell Name   V79
Profile   fibroblast Other Name   V79
Animal   hamster, Chinese Strain   Chinese
Genus   Cricetulus Species   griseus
Sex   M Age   
Identity    Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   lung
Life Span    Crisis PDL   
Morphology    Character   lung
Classify    Established by   Elkind,M.M.
Registered by   Kuroki,T. Regulation for Distribution   
Comment   Spontaneous mutation rate is relatively high. Year   
Medium   Eagle's minimal essential medium with 5% or 10% of fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.05% trypsin.
Cell Number on Passage   8x10^4 cells/ml. Race   
CO2 Conc.   5 % Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2796
Reference
Pubmed id:6935177 Mutagenesis assays with mammalian cells.
Kuroki T,Abbondandolo A,Drevon C,Huberman E,Laval F
IARC Monogr Eval Carcinog Risk Chem Hum Suppl. 1980;(2 Suppl):107-33
Pubmed id:4361348 Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Kuroki T
Exp Cell Res. 1973 Jul;80(1):55-62
Pubmed id:13901497 The evaluation of x-ray-induced chromosome aberrations in cell-cultures of the Chinese hamster.
GREENBLATT CL
Int J Radiat Biol Relat Stud Phys Chem Med. 1961 Nov;4():185-210
Pubmed id:13726391 Radiation response of mammalian cells grown in culture. 1. Repair of X-ray damage in surviving Chinese hamster cells.
ELKIND MM,SUTTON H
Radiat Res. 1960 Oct;13():556-93
Pubmed id:13576097 Observations on the chromosomes of Chinese hamster cells in tissue culture.
FORD DK,YERGANIAN G
J Natl Cancer Inst. 1958 Aug;21(2):393-425

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LOT Information

Cell No.   JCRB0603 Cell Name   
LOT No.   10012012 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/ml will be appropriate. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 14 hrs Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   
LOT No.   11242010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 2 - 3 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.2% trypsin and 0.02% EDTA
Doubling Time   approx. 13 hrs Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   96.2 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   
LOT No.   11272009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 0.5 - 1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   94.1 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   
LOT No.   10092007 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat.# 10099, lot 484375) Temperature   37 C
Cell Density at Seeding   2 - 5 x 10^4 cells/ml Methods for Passages   0.05% trypsin and 0.02% EDTA
Doubling Time   14 - 20 hrs Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   97.1 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   
LOT No.   07062005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   6 - 7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   31 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   
LOT No.   08272003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum (FCS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   1 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. At thawing plate one ampoule contents to a 75 cm2 flask.
Doubling Time   21 hrs Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown ( >P9) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   
LOT No.   03242000 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   2 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T. = ca 17 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P10* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FCS-EMEM CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   V79
LOT No.   112386 Lot Specification   distribution
Medium   MEM + 5-10% FCS Temperature   37 C
Cell Density at Seeding   split ratio=1/10 - 1/3 Methods for Passages   0.02% EDTA and 0.05% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P5* PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   
LOT No.   062696 Lot Specification   distribution
Medium   Eagle's MEM with 10% fetal calf serum (FCS lot; BioWhittaker 3M0581) Temperature   37 C
Cell Density at Seeding   4-9 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   20-24hr Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   92.3 Antibiotics Used   free
Passage Number   P3* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as Chinese hamster by NP, G6PD, MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   
LOT No.   930424 Lot Specification   distribution
Medium   MEM + 5-10% FCS Temperature   37
Cell Density at Seeding    Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   kanamycin 60 ug/ml
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
RFLP    Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB0603 Cell Name   V79
LOT No.   07292015 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 18 hrs. Cell Number in Vial (cells/1ml)   4.4 x 10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as Chinese hamster by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   99
Additional information   
Cell No.   JCRB0603 Cell Name   V79
LOT No.   10032016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding    2 - 3 x 10^4 cells/ml will be appropriate. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   3.8 x 10^6
Viability at cell freezing (%)   94.7 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94.7
Additional information   
Cell No.   JCRB0603 Cell Name   V79
LOT No.   03062018 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding    2 - 3 x 10^4 cells/ml will be appropriate. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   7.5 x 10^5
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   95.2
Additional information   
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