JCRB0606 HeLa.P3(S)
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0606 | Cell Name | HeLa.P3(S) |
|---|---|---|---|
| Profile | HeLa cell derived, can grow in a serum-free medium | Other Name | HeLa.P3(S) |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 31 |
| Identity | Tissue for Primary Cancer | uterine cervix | |
| Case history | serum free cell line established by Katsuta but later cells have been maintained with serum medium. | Metastasis | |
| Tissue Metastasized | Genetics | cervix | |
| Life Span | Crisis PDL | ||
| Morphology | Character | cervix, grow in serum free medium but later serum was used to maintain this cell line which is different from the serum free cell culture. | |
| Classify | Established by | Katsuta,H. | |
| Registered by | Kuroki,T. | Regulation for Distribution | |
| Comment | Year | 1985 | |
| Medium | DM160 medium with 10 % fetal bovine serum | Methods for Passages | Harvest cells with rubber policeman. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5 % | Tissue Sampling | cervix |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:9892199 | Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Macville M,Schröck E,Padilla-Nash H,Keck C,Ghadimi BM,Zimonjic D,Popescu N,Ried T Cancer Res. 1999 Jan 1;59(1):141-50 |
| Pubmed id:2163633 | Cytosol type electrolyte medium suitable for long term growth of human cells with very low membrane potential. Kagawa Y,Takaoka T,Hamamoto T,Ohta T Biochem Biophys Res Commun. 1990 Jun 29;169(3):1217-21 |
| Pubmed id:14404771 | Cultivation of strain HeLa cells (human cervical carcinoma) in the simplified replicate tissue culture. KATSUTA H,TAKAOKA T,KUWABARA H,KUWABARA S Jpn J Exp Med. 1959 Oct;29():493-505 |